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Titolo:
The CRK3 protein kinase is essential for cell cycle progression of Leishmania Mexicana
Autore:
Hassan, P; Fergusson, D; Grant, KM; Mottram, JC;
Indirizzi:
Univ Glasgow, Wellcome Ctr Mol Parasitol, Anderson Coll, Glasgow G11 6NU, Lanark, Scotland Univ Glasgow Glasgow Lanark Scotland G11 6NU ow G11 6NU, Lanark, Scotland
Titolo Testata:
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
fascicolo: 2, volume: 113, anno: 2001,
pagine: 189 - 198
SICI:
0166-6851(20010406)113:2<189:TCPKIE>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
BREAST-CARCINOMA CELLS; HISTONE H1 KINASE; DEPENDENT KINASES; TRYPANOTHIONE REDUCTASE; CDC2 KINASE; CHEMICAL INHIBITORS; TRYPANOSOMA-CRUZI; FISSION YEAST; HUMAN CDK2; GENE;
Keywords:
Leishmania; cyclin-dependent kinase; cell cycle; flavopiridol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Mottram, JC Univ Glasgow, Wellcome Ctr Mol Parasitol, Anderson Coll, 56 Dumbarton Rd, Glasgow G11 6NU, Lanark, Scotland Univ Glasgow 56 Dumbarton Rd Glasgow Lanark Scotland G11 6NU d
Citazione:
P. Hassan et al., "The CRK3 protein kinase is essential for cell cycle progression of Leishmania Mexicana", MOL BIOCH P, 113(2), 2001, pp. 189-198

Abstract

The Leishmania mexicana CRK3 gene encodes a cdc2-related protein kinase with activity towards histone H1. Attempts to disrupt both alleles of CRK3 inthe promastigote life-cycle stage resulted in changes in cell ploidy, which were avoided only when an extra copy of CRK3 was expressed From an episome. This provides strong evidence that CRK3 is essential to L. mexicana. Thecyclin-dependent kinase specific inhibitor flavopiridol inhibited affinitypurified histidine tagged CRK3 (CRK3his) with an IC50, value of 100 nM andinhibited in vitro growth of L. mexicana promastigotes. Incubation of promastigotes with 2.5 muM flavopiridol for 24 h led to cell cycle arrest with an accumulation of 95% of cells in G2 or early mitosis (G2/M). Release fromcell cycle arrest resulted in a semi-synchronous re-entry into the cell cycle; samples taken at 2, 4, and 6 h after release from the block were enriched for cells in G1 (68%). S-phase (70%), and G2/M phase (61%), respectively. This method of synchronisation was used to show that the majority of CRK3his activity towards the substrate histone H1 was present at G2/M. These data suggest that CRK3 has an essential role in controlling cell cycle progression at the G2/M-phase transition in L. mexicana promastigotes. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:18:30