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Titolo:
Proteolytic cleavage of staphylococcal exoproteins analyzed by two-dimensional gel electrophoresis
Autore:
Kawano, Y; Kawagishi, M; Nakano, M; Mase, K; Yamashino, T; Hasegawa, T; Ohta, M;
Indirizzi:
Nagoya Univ, Grad Sch Med, Dept Mol Bacteriol, Showa Ku, Aichi 4668550, Japan Nagoya Univ Aichi Japan 4668550 acteriol, Showa Ku, Aichi 4668550, Japan Meijyo Univ, Fac Agr, Microbiol Lab, Aichi 4688502, Japan Meijyo Univ Aichi Japan 4688502 Agr, Microbiol Lab, Aichi 4688502, Japan Nagoya Womens Univ, Fac Domest Sci, 4678610 Aichi, Japan Nagoya Womens Univ Aichi Japan 4678610 Domest Sci, 4678610 Aichi, Japan
Titolo Testata:
MICROBIOLOGY AND IMMUNOLOGY
fascicolo: 4, volume: 45, anno: 2001,
pagine: 285 - 290
SICI:
0385-5600(2001)45:4<285:PCOSEA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-CLONING; AUREUS; PROTEASE; IDENTIFICATION; EXPRESSION; GENE; AGR; METALLOPROTEASE; ACTIVATION; VIRULENCE;
Keywords:
staphylococcal exoproteins; extracellular proteases; two-dimensional polyacrylamide-gel electrophoresis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Ohta, M Nagoya Univ, Grad Sch Med, Dept Mol Bacteriol, Showa Ku, 65 Tsurumai Cho, Aichi 4668550, Japan Nagoya Univ 65 Tsurumai Cho Aichi Japan 4668550 hi 4668550, Japan
Citazione:
Y. Kawano et al., "Proteolytic cleavage of staphylococcal exoproteins analyzed by two-dimensional gel electrophoresis", MICROB IMMU, 45(4), 2001, pp. 285-290

Abstract

Extracellular proteases of Staphylococcus aureus are emerging as potentialvirulence factors that are relevant to the pathogenicity of staphylococcalinfections. These proteases may also be involved in the proteolytic cleavage of other exoproteins released from this organism. To define the target exoproteins and their sites of cleavage by proteases, high-resolution two-dimensional polyaerylamide gel electrophoresis followed by N-terminal amino acid sequencing of exoprotein spots was performed. Two to three hundred exoprotein spots were detected at the early-stationary phase of cultures of S. aureus NCTC8325, and then at the late-stationary stage most of these high molecular protein spots became invisible due to further proteolytic degradation. As the result of N-terminal analysis, lipase, triacylglycerol lipase, orf619 protein and orf388 protein were detected as multiple spots at the early-stationary phase. We found that these exoproteins were cleaved at 3, 7,4 and 4 different sites, respectively, by proteases. According to the M.W.and pI of each peptide spot obtained from the gel and their matches with calculated values in addition to their N-terminal sequences, we showed that the positions of putative peptides resulted from proteolytic cleavage of these proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 01:09:50