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Titolo:
Intrinsic fluorescence of enzymes and fluorescence of chemically modified enzymes for analytical purposes: a review
Autore:
Galban, J; Andreu, Y; Sierra, JF; de Marcos, S; Castillo, JR;
Indirizzi:
Univ Zaragoza, Fac Sci, Dept Analyt Chem, Analyt Spect & Sensors Grp, E-50009 Zaragoza, Spain Univ Zaragoza Zaragoza Spain E-50009 ensors Grp, E-50009 Zaragoza, Spain
Titolo Testata:
LUMINESCENCE
fascicolo: 2, volume: 16, anno: 2001,
pagine: 199 - 210
SICI:
1522-7235(200103/04)16:2<199:IFOEAF>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENZYMATIC FLUOROMETRIC ASSAY; WATER-SOLUBLE CARBODIIMIDE; HYDROGEN-PEROXIDE; GLUCOSE-OXIDASE; HORSERADISH-PEROXIDASE; BIOSENSOR; SERUM; LACTATE; SENSOR; DIAGNOSIS;
Keywords:
enzyme; intrinsic fluorescence; chemically modified enzymes; review;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Galban, J Univ Zaragoza, Fac Sci, Dept Analyt Chem, Analyt Spect & SensorsGrp, E-50009 Zaragoza, Spain Univ Zaragoza Zaragoza Spain E-50009 , E-50009 Zaragoza, Spain
Citazione:
J. Galban et al., "Intrinsic fluorescence of enzymes and fluorescence of chemically modified enzymes for analytical purposes: a review", LUMINESCENC, 16(2), 2001, pp. 199-210

Abstract

In recent years our research group has developed new alternatives for fluorescence enzymatic determinations. First, we observed that the intrinsic fluorescence of enzymes changes during enzymatic reactions, proportionally tothe substrate concentration, avoiding the combination of the enzymatic reaction with a fluorophore-involving reaction. The main disadvantage of this method is that the excitation and emission wavelengths of the enzymes are in the UV region of the spectrum. An alternative to overcome this problem consisted of covalently bonding the enzyme to a fluorophore. In this paper, an overview is given of all of the applications and future developments on both types of alternatives that we have developed. Apart from the analyticalcharactersitics of the methods, we have also reviewed ail of the information about mathematical models we have elaborated to date. Copyright (C) 2001John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:31:53