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Titolo:
Importance of the ionization states of m-THPC for its HPLC fluorescence detection
Autore:
Desroches, MC; Bourdon, O; Morokro, Y; Chaminade, P; Blais, J; Prognon, P; Kasselouri, A;
Indirizzi:
Univ Paris 11, Fac Pharm, Chim Analyt Lab, F-92296 Chatenay Malabry, France Univ Paris 11 Chatenay Malabry France F-92296 6 Chatenay Malabry, France Univ Paris 06, Lab Physicochim Biomol & Cellulare, CNRS, ESA 7033, F-75252Paris 05, France Univ Paris 06 Paris France 05 e, CNRS, ESA 7033, F-75252Paris 05, France
Titolo Testata:
LUMINESCENCE
fascicolo: 2, volume: 16, anno: 2001,
pagine: 173 - 178
SICI:
1522-7235(200103/04)16:2<173:IOTISO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; PHOTODYNAMIC THERAPY; HUMAN-PLASMA; 5,10,15,20-TETRA-(M-HYDROXYPHENYL)CHLORIN; PHARMACOKINETICS; TEMOPORFIN; CANCER;
Keywords:
PDT; m-THPC; HPLC; fluorescence; post-column reaction; pK(A);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Desroches, MC Univ Paris 11, Fac Pharm, Chim Analyt Lab, 5 Rue JB Clement,F-92296 Chatenay Malabry, France Univ Paris 11 5 Rue JB Clement Chatenay Malabry France F-92296
Citazione:
M.C. Desroches et al., "Importance of the ionization states of m-THPC for its HPLC fluorescence detection", LUMINESCENC, 16(2), 2001, pp. 173-178

Abstract

An original, rapid and sensitive high-performance liquid chromatographic (HPLC) method has been developed for the detection of 5, 10, 15, 20 tetra-meso-hydroxyphenylchlorine (m-THPC), a photosensitizer used in the photodynamic therapy (PDT) of cancers. Chromatographic separation was carried but on a C-8 Zorbax(R) column (80 x 4 mm, 5 mum). The mobile phase was an ethanol/aqueous sulphuric acid, pH 2.0 (65/35 v/v), in an isocratic mode yielding to rapid analysis (3.1 min) with narrow peaks. As the fluorescence intensitywas found to be highly pH-dependent and to increase with pH values, a post-column device prior to the fluorescence detection (lambda (exc) = 423 nm, lambda (em) = 650 nm) was used to allow the addition of a 0.05 mol/L Na2HPO4 solution to the mobile phase. Compared to standard conditions, a 300% increase of the fluorescence intensity was obtained for optimized operating conditions using experimental design. The validation of this analytical method showed;hat the response function was linear for concentrations up to 1000mug/L (1.47 x 10(-6) mol/L) with a detection limit of 188 pg (S/N = 3). Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 07:34:04