Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Reporter cell lines are useful tools for monitoring biological activity ofnuclear receptor ligands
Autore:
Balaguer, P; Boussioux, AM; Demirpence, E; Nicolas, JC;
Indirizzi:
INSERM, U439, F-34090 Montpellier, France INSERM Montpellier France F-34090 ERM, U439, F-34090 Montpellier, France
Titolo Testata:
LUMINESCENCE
fascicolo: 2, volume: 16, anno: 2001,
pagine: 153 - 158
SICI:
1522-7235(200103/04)16:2<153:RCLAUT>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
BREAST-CANCER CELLS; RETINOIC ACID;
Keywords:
stable transfection; firefly luciferase; chimaeric nuclear receptor; nuclear receptors ligands; xeno-oestrogens; subtype-selective retinoids;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Balaguer, P INSERM, U439, 70 Rue Navacelles, F-34090 Montpellier, France INSERM 70 Rue Navacelles Montpellier France F-34090 r, France
Citazione:
P. Balaguer et al., "Reporter cell lines are useful tools for monitoring biological activity ofnuclear receptor ligands", LUMINESCENC, 16(2), 2001, pp. 153-158

Abstract

To characterize the specificity of synthetic compounds for nuclear receptors, we established stable cell lines expressing the luciferase gene and different wild-type or chimaeric receptors. MCF-7 cells, which express the oestrogen receptor alpha (ER alpha), and HeLa cells, which do not express the oestrogen receptor, were transfected with a plasmid containing the luciferase gene downstream from a minimum promoter (beta -globin) and an oestrogen-responsive element, generating the MELN and the HELN cell lines, respectively. MELN cells enabled the detection of compounds that bind to the ER alphaor interfere with its pathway. HELN cells were used to establish stable transfectants expressing different nuclear receptors containing the DNA-binding domain of the oestrogen receptors. We thus established ER alpha or ER beta reporter cell lines by transfecting ER alpha or ER beta expression plasmids, and also retinoic acid receptor alpha, beta or gamma reporter cell lines by transfecting the chimaene RAR gene, in which the DNA-binding domain was replaced by the ER alpha DNA-binding domain. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 10:53:10