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Titolo:
Properties of the Na+-H+ exchanger in renal microvillus membrane vesicles (Reprinted from Am. J. Physiol, vol 238, pg F461-F469, 1980)
Autore:
Kinsella, JL; Aronson, PS;
Indirizzi:
Yale Univ, Sch Med, Dept Med, New Haven, CT 06510 USA Yale Univ New HavenCT USA 06510 h Med, Dept Med, New Haven, CT 06510 USA Yale Univ, Sch Med, Dept Physiol, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 d, Dept Physiol, New Haven, CT 06510 USA
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
fascicolo: 5, volume: 12, anno: 2001,
pagine: 1085 - 1095
SICI:
1046-6673(200105)12:5<1085:POTNEI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRUSH-BORDER MEMBRANES; RAT SMALL-INTESTINE; DEPENDENT TRANSPORT; INTRACELLULAR PH; D-GLUCOSE; MUSCLE-FIBERS; MECHANISM; GRADIENT;
Keywords:
lithium; proximal tubule; renal cortex; acidification; bicarbonate transport;
Tipo documento:
Reprint
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Kinsella, JL Yale Univ, Sch Med, Dept Med, New Haven, CT 06510 USA Yale Univ New Haven CT USA 06510 ed, New Haven, CT 06510 USA
Citazione:
J.L. Kinsella e P.S. Aronson, "Properties of the Na+-H+ exchanger in renal microvillus membrane vesicles (Reprinted from Am. J. Physiol, vol 238, pg F461-F469, 1980)", J AM S NEPH, 12(5), 2001, pp. 1085-1095

Abstract

Transport of Na+ and H+ was evaluated in brush border membrane vesicles isolated from the rabbit renal cortex. Na+ transport was assayed by a rapid filtration technique; H+ transport was monitored with 5,5-dimethoxazolidine-2,4-dione by flow dialysis. Uphill Na+ uptake was induced by imposition of an in > out H+ gradient, and uphill H+ efflux by imposition of an out > in Na+ gradient, consistent with the action of a Na+-H+ exchanger. The uptake of Na+ was electroneutral either in the presence or absence of a H+ gradient, indicating a fixed 1:1 stoichiometry for the exchange process. Na+ transport was saturable and inhibited by Li+ and NH4+ but not by K-, Rb+, Cs+, or choline. Uphill H+ efflux was induced by imposition of an out > in Li+ gradient. Neither the uptake of Na+ nor H+ efflux were influenced by out > ingradients of Cl- compared to gradients of SCN- or SO42-. If transport systems mediating Na+-Cl- co-transport and/or Cl--OH- exchange are present in the microvillus membrane, their respective rates must be slow compared to the rate of Na+-H+ exchange. Transport of Na+ was inhibited by harmaline and amiloride, but not by acetazolamide, furosemide, or 4-acetamido-4'-isothiocyanostilbene-2,2'-disulfonate. We conclude that isolated renal microvillus membranes contain a tightly coupled Na+-H+ exchanger that may play an important role in proximal tubular acidification.

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Documento generato il 04/04/20 alle ore 06:28:33