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Titolo:
Cisplatin decreases the abundance of aquaporin water channels in rat kidney
Autore:
Kim, SW; Lee, JU; Nah, MY; Kang, DG; Ahn, KY; Lee, HS; Choi, KC;
Indirizzi:
Chonnam Natl Univ, Sch Med, Dept Physiol, Kwangju 501746, South Korea Chonnam Natl Univ Kwangju South Korea 501746 Kwangju 501746, South Korea Chonnam Natl Univ, Sch Med, Dept Internal Med, Kwangju 501746, South KoreaChonnam Natl Univ Kwangju South Korea 501746 Kwangju 501746, South Korea Chonnam Natl Univ, Sch Med, Dept Anat, Kwangju 501746, South Korea ChonnamNatl Univ Kwangju South Korea 501746 Kwangju 501746, South Korea Wonkwang Univ, Profess Grad Sch Oriental Med, Iksan, South Korea Wonkwang Univ Iksan South Korea ad Sch Oriental Med, Iksan, South Korea
Titolo Testata:
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
fascicolo: 5, volume: 12, anno: 2001,
pagine: 875 - 882
SICI:
1046-6673(200105)12:5<875:CDTAOA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENYLATE-CYCLASE; COLLECTING DUCT; EXPRESSION; ACTIVATION; MEMBRANE; FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, JU Chonnam Natl Univ, Sch Med, Dept Physiol, 5 Hak Dong, Kwangju 501746, South Korea Chonnam Natl Univ 5 Hak Dong Kwangju South Korea 501746 uth Korea
Citazione:
S.W. Kim et al., "Cisplatin decreases the abundance of aquaporin water channels in rat kidney", J AM S NEPH, 12(5), 2001, pp. 875-882

Abstract

The present study examined whether the cisplatin-induced urinary concentration defect can be related to an altered regulation of aquaporin (AQP) water channels in the kidney. Cisplatin (8 mg/kg) was injected intraperitoneally into male Sprague-Dawley rats. The control group was without cisplatin treatment. Four d later, the expression of AQP1, AQP2, and AQP3 proteins was determined in the kidney. To specify further the primary point of derangement in the pathway that activates the arginine vasopressin-mediated AQP channels, different components of adenylyl cyclase complex were examined separately. The cisplatin treatment caused a polyuric renal failure in association with decreases of free water reabsorption. The expression of AQP1 and AQP2 was decreased in the cortex, the outer medulla, and the inner medulla, whereas that of AQP3 was decreased in the outer medulla and the inner medulla. The expression of AQP2 proteins in the apical membrane-enriched fraction decreased in parallel with that in the subapical vesicle-enriched fraction,indicating a preserved targeting. Immunohistochemistry of the outer medulla also revealed that cisplatin decreased immunoreactivity for AQP1, AQP2, and AQP3. The arginine vasopressin-evoked generation of cyclic adenosine monophosphate was attenuated by cisplatin, being most prominent in the outer medulla. However, the cyclic adenosine monophosphate generation in response to forskolin was not affected, whereas that to sodium fluoride was diminished significantly. Cisplatin also decreased the expression of Gs alpha proteins in the outer medulla and the inner medulla. These results suggest that a reduced expression of AQP water channels accounts at least in part for the cisplatin-induced urinary concentration defect.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 21:58:51