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Titolo:
Bilirubin-IX beta is a marker of meconium, like zinc coproporphyrin
Autore:
Aziz, S; Leroy, P; Servaes, R; Eggermont, E; Fevery, J;
Indirizzi:
Catholic Univ Louvain, Univ Hosp Gasthuisberg, Lab Hepatol, B-3000 Louvain, Belgium Catholic Univ Louvain Louvain Belgium B-3000 ol, B-3000 Louvain, Belgium Catholic Univ Louvain, Univ Hosp Gasthuisberg, Dept Paediat, B-3000 Louvain, Belgium Catholic Univ Louvain Louvain Belgium B-3000 at, B-3000 Louvain, Belgium
Titolo Testata:
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION
fascicolo: 3, volume: 32, anno: 2001,
pagine: 287 - 292
SICI:
0277-2116(200103)32:3<287:BBIAMO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; HOMOZYGOUS GUNN RATS; BILIVERDIN-IX; ISOMERS; EXCRETION; JAUNDICE; PURIFICATION; REDUCTASE; PIGMENTS; BILE;
Keywords:
meconium; bilirubin-IX alpha; bilirubin-IX beta; coproporphyrin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Fevery, J Catholic Univ Louvain, Univ Hosp Gasthuisberg, Lab Hepatol, B-3000 Louvain, Belgium Catholic Univ Louvain Louvain Belgium B-3000 Louvain, Belgium
Citazione:
S. Aziz et al., "Bilirubin-IX beta is a marker of meconium, like zinc coproporphyrin", J PED GASTR, 32(3), 2001, pp. 287-292

Abstract

Background: Because meconium accumulates continuously in the fetal intestine, analysis of the postnatally excreted material could yield important information of intrauterine metabolism and maturation. Therefore, a study of the bilirubin pigments in meconium and in the first neonatal stools was carried out. Methods: Meconium and stools from 37 neonates of various gestational ages were collected carefully, and stored at -20 degreesC, protected by aluminium foil. Samples were defrosted, vortex mixed with an equal amount of dimethyl sulfoxide, centrifuged, and submitted to analysis by high-pressure liquid chromatography using newly developed methods to identify and to quantitate the bilirubin-IX alpha, -IX beta, -IX gamma, and -IX delta isomers. In addition, samples were also submitted to diazo coupling with ethyl anthranilate. Total coproporphyrins and zinc coproporphyrin were assayed for comparison. Results: Unconjugated bilirubin-IX alpha and -IX beta were detected in meconium but not the -IX gamma or the -IX delta isomer. Bilirubin-IX beta was the predominant pigment and comprised 63% to 96% of the unconjugated bilirubins in the first sample of meconium excreted. Its amount decreased rapidlyduring the first 5 days in full-term newborns, but this occurred more slowly in preterm neonates, especially in those with a gestational age less than 30 weeks. The decrease of bilirubin-IX beta over time correlated with that of coproporphyrin. Conclusions: Bilirubin-IX beta is the prevailing bile pigment in the firstexcreted sample of meconium. It gradually decreases after birth and can beconsidered a biochemical marker of meconium, like zinc coproporphyrin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 21:59:20