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Titolo:
Comparative analysis of human papillomavirus infections in cervical scrapes and biopsy specimens by general SPF10PCR and HPV genotyping
Autore:
Quint, WGV; Scholte, G; van Doorn, LJ; Kleter, B; Smits, PHM; Lindeman, J;
Indirizzi:
Delft Diagnost Lab, NL-2625 AD Delft, Netherlands Delft Diagnost Lab Delft Netherlands NL-2625 AD 25 AD Delft, Netherlands Slotervaart Hosp, Dept Pathol, Amsterdam, Netherlands Slotervaart Hosp Amsterdam Netherlands t Pathol, Amsterdam, Netherlands Slotervaart Hosp, Dept Mol Biol, Amsterdam, Netherlands Slotervaart Hosp Amsterdam Netherlands Mol Biol, Amsterdam, Netherlands
Titolo Testata:
JOURNAL OF PATHOLOGY
fascicolo: 1, volume: 194, anno: 2001,
pagine: 51 - 58
SICI:
0022-3417(200105)194:1<51:CAOHPI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; LINE PROBE ASSAY; BROAD-SPECTRUM; CLINICAL-EVALUATION; FOLLOW-UP; DNA; PCR; HYBRIDIZATION; WOMEN; NEOPLASIA;
Keywords:
HPV; DNA; PCR; LiPA; genotyping; multiple infections;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Quint, WGV Delft Diagnost Lab, Reinier de Graafweg 7, NL-2625 AD Delft, Netherlands Delft Diagnost Lab Reinier de Graafweg 7 Delft Netherlands NL-2625 AD
Citazione:
W.G.V. Quint et al., "Comparative analysis of human papillomavirus infections in cervical scrapes and biopsy specimens by general SPF10PCR and HPV genotyping", J PATHOLOGY, 194(1), 2001, pp. 51-58

Abstract

Human papillomavirus (HPV) can be detected by DNA amplification from clinical samples. The aim of the present study was to compare the HPV status in both cervical scrape and biopsy specimens obtained from 174 patients, usingthe recently developed broad spectrum SPF10 PCR-LiPA method. The detectionrate of HPV in these materials was determined and the spectrum of HPV genotypes was compared. Cervical scrapes and biopsy specimens were obtained, either on the same day. (group I), or with an interval of up to almost 2 years (group II, mean interval 97 days, range 1-469 days). HPV DNA was amplified hy SPF10 PCR and detected in a microtitre plate hybridization assay. Of the HPV-positive cases, the genotype was determined by reverse hybridizationof the same SPF10 amplimer on a line probe assay (LiPA), discriminating between HPV genotypes 6, 11, 16, 18, 31, 33-35, 39, 40, 42,-45, 51-54, 56, 58, 59, 66, 68, 70, and 74. The results showed that the detection rate and the spectrum of HPV genotypes in cervical scrapes and the corresponding biopsy specimens were highly comparable in both patient groups, even when multiple genotypes were present. In both groups, multiple HPV genotypes were morefrequently detected in cervical scrapes than in the corresponding biopsy. specimens. In conclusion, HPV infection can be diagnosed in cervical scrapes and biopsy specimens using the SPF10 PCR-LiPA system. Analysis of cervical scrapes accurately reflects the spectrum of HPV genotypes in the patient's cervical region, even with a sampling interval between the cervical scrape and the biopsy specimen. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:16:48