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Titolo:
Differential cellular accumulation of connective tissue growth factor defines a subset of reactive astrocytes, invading fibroblasts, and endothelial cells following central nervous system injury in rats and humans
Autore:
Schwab, JM; Beschorner, R; Nguyen, TD; Meyermann, R; Schluesener, HJ;
Indirizzi:
Univ Tubingen, Sch Med, Inst Brain Res, D-72076 Tubingen, Germany Univ Tubingen Tubingen Germany D-72076 in Res, D-72076 Tubingen, Germany
Titolo Testata:
JOURNAL OF NEUROTRAUMA
fascicolo: 4, volume: 18, anno: 2001,
pagine: 377 - 388
SICI:
0897-7151(200104)18:4<377:DCAOCT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANGIOGENESIS IN-VIVO; BRAIN INJURY; SPINAL-CORD; FACTOR-BETA; CNS INJURY; EXPRESSION; FACTOR-BETA(1); PROLIFERATION; DEGENERATION; TGF-BETA-1;
Keywords:
astrocyte activation; blood-brain barrier; glial scar; secondary injury; tissue remodeling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Schwab, JM Univ Tubingen, Sch Med, Inst Brain Res, Calwer Str 3, D-72076 Tubingen, Germany Univ Tubingen Calwer Str 3 Tubingen Germany D-72076 n, Germany
Citazione:
J.M. Schwab et al., "Differential cellular accumulation of connective tissue growth factor defines a subset of reactive astrocytes, invading fibroblasts, and endothelial cells following central nervous system injury in rats and humans", J NEUROTRAU, 18(4), 2001, pp. 377-388

Abstract

In brain injury, the primary trauma is followed by a cascade of cellular and molecular mechanisms resulting in secondary injury and scar formation. Astrogliosis and expression of transforming growth factor beta (TGF-beta) are key components of scar formation. A cytokine mediating the effects of TGF-beta is connective tissue growth factor (CTGF), a fibrogenic peptide encoded by an immediate early gene with suggested roles in tissue regeneration and aberrant deposition of extracellular matrix. In order to investigate CTGF in traumatic lesions, we evaluated 20 human brains with traumatic brain injury (TBI) and 18 rat brains with stab wound injury. Compared to remote areas and unaltered control brains, CTGF(+) cells accumulated in border zonesof the traumatic lesion site (p < 0.0001). In the direct peri-lesional rim, CTGF expression was confined to invading vimentin(+), GFAP(-) fibroblastoid cells, endothelial and smooth muscle cells of laminin(+) vessels, and GFAP(+) reactive astrocytes. In the direct peri-lesional rim, CTGF(+) astrocytes (>80%) co-expressed the activation associated intermediate filaments nestin and vimentin. In injured rat brains, numbers of CTGF(+) cells peaked at day 3 and 7 and decreased to almost base level 3 weeks postinjury, whereas in humans, CTGF(+) cells remained persistently elevated up to 6 months (p< 0.0001). The restricted accumulation of CTGF(+)-reactive astrocytes and CTGF(+) fibroblastoid cells lining the adjacent laminin(+) basal lamina suggests participation of these cells in scar formation. Furthermore, peri-lesional upregulation of endothelial CTGF expression points to a role in blood-brain barrier function and angiogenesis. In addition, CTGF appears to be asensitive marker of early astrocyte activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 06:23:08