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Titolo:
Protein tyrosine kinase regulates Fas-mediated apoptosis in human BCG-infected monocytes
Autore:
Mendez-Samperio, P; Vazquez, A; Morales, V; Palma, J;
Indirizzi:
IPN, Escuela Nacl Ciencias Biol, Dept Immunol, Mexico City 11340, DF, Mexico IPN Mexico City DF Mexico 11340 pt Immunol, Mexico City 11340, DF, Mexico
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 4, volume: 21, anno: 2001,
pagine: 209 - 217
SICI:
1079-9907(200104)21:4<209:PTKRFA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACILLUS-CALMETTE-GUERIN; PROGRAMMED CELL-DEATH; TUMOR NECROSIS FACTOR; MYCOBACTERIUM-AVIUM; DIFFERENTIAL EXPRESSION; HUMAN MACROPHAGES; PERIPHERAL-BLOOD; TNF RECEPTOR; ACTIVATION; ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Mendez-Samperio, P IPN, Escuela Nacl Ciencias Biol, Dept Immunol, Carpio &Plan Ayala, MexicoCity 11340, DF, Mexico IPN Carpio & Plan Ayala Mexico City DF Mexico 11340 ico
Citazione:
P. Mendez-Samperio et al., "Protein tyrosine kinase regulates Fas-mediated apoptosis in human BCG-infected monocytes", J INTERF CY, 21(4), 2001, pp. 209-217

Abstract

Apoptosis of monocytes/macrophages has emerged as a central regulatory event in the defense against mycobacterial infections. The involvement of protein tyrosine kinases (PTK) in Fas-mediated apoptosis in T cells is well established, but the possible role of PTK in Fas-dependent death of human bacillus Calmette-Guerin (BCG)-infected monocytes remains unclear. Here, we first examined the expression and function of Fas on BCG-infected human monocytes by flow cytometry. The results demonstrated that BCG-infected monocytesexpressed significant Fas protein levels. In addition, engagement of the Fas antigen with its agonistic antibody (Ab) resulted in apoptosis of monocytes, as monitored by DNA analysis and fluorescence-activated cell sorter (FACS) analysis. The apoptotic action of Fas was suppressed significantly by genistein, indicating a role for PTK in this death process. Consistent withthis observation, herbimycin A and tyrphostin, two selective tyrosine kinase inhibitors with different mechanisms of action, effectively inhibited Fas-mediated apoptosis of BCG-infectcd monocytes, as demonstrated by DNA content analysis. Moreover, we confirmed the effect of genistein, herbimycin A,and tyrphostin by examining apoptosis with the terminal transferase dUTP nick end-labeling (TUNEL) assay. Collectively, these data demonstrate that Fas-induced apoptosis may represent an important mechanism for eliminating BCG-activated human monocytes and that this apoptosis is due, at least in part, to signaling via a PTK pathway.

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Documento generato il 03/04/20 alle ore 04:31:49