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Titolo:
Late temporal gene expression from the human cytomegalovirus pp28US (UL99)promoter when integrated into the host cell chromosome
Autore:
Wu, J; ONeill, J; Barbosa, MS;
Indirizzi:
Calgene, Signal Res Div, San Diego, CA 92121 USA Calgene San Diego CA USA92121 e, Signal Res Div, San Diego, CA 92121 USA
Titolo Testata:
JOURNAL OF GENERAL VIROLOGY
, volume: 82, anno: 2001,
parte:, 5
pagine: 1147 - 1155
SICI:
0022-1317(200105)82:<1147:LTGEFT>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMEDIATE-EARLY GENE; HERPESVIRUS MACROMOLECULAR-SYNTHESIS; IE2 86-KILODALTON PROTEIN; SIMPLEX VIRUS TYPE-1; REGULATED EXPRESSION; ENDOTHELIAL-CELLS; HUMAN-FIBROBLASTS; BINDING PROTEIN; VIRAL-PROTEINS; CAP SITE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Wu, J Calgene, Signal Res Div, 5555 Oberlin Dr, San Diego, CA 92121 USA Calgene 5555 Oberlin Dr San Diego CA USA 92121 n Diego, CA 92121 USA
Citazione:
J. Wu et al., "Late temporal gene expression from the human cytomegalovirus pp28US (UL99)promoter when integrated into the host cell chromosome", J GEN VIROL, 82, 2001, pp. 1147-1155

Abstract

Toward understanding the temporal regulation of human cytomegalovirus (HCMV) late genes, we studied the regulation of the late gene promoter (pp28US,UL99) when outside the context of the viral genome and its response to theimmediate early (IE) proteins. Expression of the luciferase reporter gene,regulated by the pp28US promoter, was synchronous with that of the endogenous viral pp28 gene, independently of whether the reporter was episomal or integrated into the glioblastoma cell line U373MG. Cotransfection of the reporter with expression vectors for each of the three major IE genes, IE72, IE86 and IE55, indicated that only IE86 transactivated the pp28US promoter. However, the magnitude of the promoter activation upon HCMV infection suggested that additional factors are also required for higher promoter activity. The promoter activation was specific to HCMV, as herpes simplex virus type 1 infection did not induce luciferase expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:10:53