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Titolo:
Discrete targeting signals direct Pmp47 to oleate-induced peroxisomes in Saccharomyces cerevisiae
Autore:
Wang, XD; Unruh, MJ; Goodman, JM;
Indirizzi:
Univ Texas, SW Med Ctr, Dept Pharmacol, Dallas, TX 75390 USA Univ Texas Dallas TX USA 75390 Ctr, Dept Pharmacol, Dallas, TX 75390 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 14, volume: 276, anno: 2001,
pagine: 10897 - 10905
SICI:
0021-9258(20010406)276:14<10897:DTSDPT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEMBRANE-PROTEIN PMP47; UBIQUITIN-CONJUGATING ENZYME; POLYMERASE CHAIN-REACTION; HANSENULA-POLYMORPHA; ENDOPLASMIC-RETICULUM; YARROWIA-LIPOLYTICA; PICHIA-PASTORIS; IMPORT; PROLIFERATION; BIOGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Goodman, JM Univ Texas, SW Med Ctr, Dept Pharmacol, 5323 Harry Hines Blvd,Dallas, TX 75390 USA Univ Texas 5323 Harry Hines Blvd Dallas TX USA 75390 75390 USA
Citazione:
X.D. Wang et al., "Discrete targeting signals direct Pmp47 to oleate-induced peroxisomes in Saccharomyces cerevisiae", J BIOL CHEM, 276(14), 2001, pp. 10897-10905

Abstract

Pmp47 is a peroxisomal membrane protein consisting of six transmembrane domains (TMDs), We previously showed that the second matrix loop containing abasic cluster of amino acids is important for peroxisomal targeting, and similar basic targeting motifs have been found in other peroxisomal membraneproteins. However, this basic cluster by itself targets to peroxisomes very poorly. We have developed a sensitive quantitative localization assay based on the targeting of Pmp47-GFP fusion proteins to identify the important elements of the basic cluster and to search for other targeting informationon Pmp47. Our data suggest that side-chain structure and position as well as charge are important for targeting by the basic cluster, Analysis of other regions of Pmp47 indicates that all TMDs except TMD2 can be eliminated or substituted without significant loss of targeting. TMD2 plus an adjacent cytoplasmic-oriented sequence is crucial for targeting. Cytoplasmic-oriented sequences from two other peroxisomal membrane proteins, ScPex15p and ScPmp22, could partially substitute for the analogous sequence in Pmp47, Targeting with high fidelity to oleate-induced peroxisomes required the followingelements: the cytoplasmic-oriented sequence and TMD2, a short matrix loop containing a basic cluster, and a membrane-anchoring TMD.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 20:26:33