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Titolo:
Its8, a fission yeast homolog of Mcd4 and Pig-n, is involved in GPI anchorsynthesis and shares an essential function with calcineurin in cytokinesis
Autore:
Yada, T; Sugiura, R; Kita, A; Itoh, Y; Lu, YB; Hong, YJ; Kinoshita, T; Shuntoh, H; Kuno, T;
Indirizzi:
Kobe Univ, Sch Med, Dept Pharmacol, Chuo Ku, Kobe, Hyogo 6500017, Japan Kobe Univ Kobe Hyogo Japan 6500017 l, Chuo Ku, Kobe, Hyogo 6500017, Japan Osaka Univ, Microbial Dis Res Inst, Dept Immunoregulat, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 regulat, Suita, Osaka 5650871, Japan Kobe Univ, Sch Med, Fac Hlth Sci, Kobe, Hyogo 6540142, Japan Kobe Univ Kobe Hyogo Japan 6540142 c Hlth Sci, Kobe, Hyogo 6540142, Japan
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 17, volume: 276, anno: 2001,
pagine: 13579 - 13586
SICI:
0021-9258(20010427)276:17<13579:IAFYHO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; SACCHAROMYCES-CEREVISIAE; SCHIZOSACCHAROMYCES-POMBE; CELL INTEGRITY; CYCLOSPORINE-A; FIRST STEP; GENE; GLYCOSYLPHOSPHATIDYLINOSITOL; BIOSYNTHESIS; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Kuno, T Kobe Univ, Sch Med, Dept Pharmacol, Chuo Ku, 7-5-1 Kusunoki Cho, Kobe, Hyogo 6500017, Japan Kobe Univ 7-5-1 Kusunoki Cho Kobe Hyogo Japan 6500017 00017, Japan
Citazione:
T. Yada et al., "Its8, a fission yeast homolog of Mcd4 and Pig-n, is involved in GPI anchorsynthesis and shares an essential function with calcineurin in cytokinesis", J BIOL CHEM, 276(17), 2001, pp. 13579-13586

Abstract

In fission yeast, calcineurin is required for cytokinesis and ion homeostasis; however, most of its physiological roles remain obscure. To identify genes that share an essential function with calcineurin, we screened for mutations that confer sensitivity to the calcineurin inhibitor FK506 and high temperature and isolated the mutant its8-1. its8(+) encodes a homolog of the budding yeast MCD4 and human Pig-n that are involved in glycosylphosphatidylinositol (GPI) anchor synthesis. Consistently, reduced inositol labelingof proteins suggested impaired GPI anchor synthesis in its8-1 mutants. Thetemperature upshift induced a further decrease in inositol labeling and caused dramatic increases in the frequency of septation in its8-1 mutants. BE49385A, an inhibitor of MCD4 and Pig-n, also increased the septation index of the wild-type cell. Osmotic stabilization suppressed these morphologicaldefects, indicating that cell wall weakness caused by impaired GPI anchor synthesis resulted in abnormal cytokinesis. Furthermore, calcineurin-deleted cells exhibited hypersensitivity to BE49385A, and FK506 exacerbated the cytokinesis defects of the its8-1 mutant, Thus, calcineurin and Its8 may share an essential function in cytokinesis and cell viability through the regulation of cell wall integrity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 10:57:29