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Titolo:
Pharmacological characterization of the CGRP receptor in the lateral line organ of Xenopus laevis
Autore:
Bailey, GP; Sewell, WF;
Indirizzi:
Massachusetts Eye & Ear Infirm, Eaton Peabody Lab Auditory Physiol, Boston, MA 02114 USA Massachusetts Eye & Ear Infirm Boston MA USA 02114 , Boston, MA 02114 USA Boston Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA Boston Univ Boston MA USA 02115 ch Med, Dept Pathol, Boston, MA 02115 USA Harvard Univ, Sch Med, Dept Otolaryngol, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 , Dept Otolaryngol, Boston, MA 02115 USA Harvard Univ, Sch Med, Program Neurosci, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 , Program Neurosci, Boston, MA 02115 USA
Titolo Testata:
JARO
fascicolo: 1, volume: 1, anno: 2000,
pagine: 82 - 88
SICI:
1525-3961(200008)1:1<82:PCOTCR>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-RELATED PEPTIDE; SUPERIOR OLIVARY COMPLEX; RAT VAS-DEFERENS; GUINEA-PIG; CALCITONIN-RECEPTOR; MULTIPLE RECEPTORS; INNER-EAR; CORONARY-ARTERY; FINE-STRUCTURE; AMYLIN;
Keywords:
cochlea; vestibular; hair cell; efferents; CGRP; metamorphic;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Sewell, WF Massachusetts Eye & Ear Infirm, Eaton Peabody Lab Auditory Physiol, 243 Charles St, Boston, MA 02114 USA Massachusetts Eye & Ear Infirm 243 Charles St Boston MA USA 02114
Citazione:
G.P. Bailey e W.F. Sewell, "Pharmacological characterization of the CGRP receptor in the lateral line organ of Xenopus laevis", JARO, 1(1), 2000, pp. 82-88

Abstract

Calcitonin gene-related peptide (CGRP) is a neurotransmitter candidate colocalized with acetylcholine in efferent fibers innervating hair cell organs. We have used the Xenopus laevis lateral line organ to investigate the responses of a hair cell organ to the CGRP family of peptides. Two isoforms ofCGRP, r alpha CGRP and r beta -CGRP, and a human analog of alpha -CGRP, h(Tyr degrees) alpha -CGRP, produced dose-dependent increases in efferent nerve fiber discharge rate with EC50 values of approximately 1 muM. Rate increases were 31.2, 18.9, and 10.3%, respectively The peptide fragment rCGRP(8-37) a selective CGRP(1) receptor antagonist, competitively inhibited the response to r alpha -CGRP. Diacetoamidomethyl cysteine CGRP (r[Cys(ACM)(2,7)]alpha -CGRP), a CGRP(2) agonist, did not change discharge rate. Rat amylindid not increase rate until very high concentrations, and then the change was less than 7%. Rat adrenomedullin produced no increase in rate. Responses to r alpha -CGRP developed after metamorphosis. No change in spontaneous discharge rate was observed until postmetamorphic day 6, and then it was only a fraction of the maximal response. This response progressively increased until postmetamorphic day 28, when it reached its maximal value. The moststraightforward interpretation of our results is that the effect of CGRP is mediated by the CGRP(1) receptor and that CGRP, of the peptides presentlyknown to exist in the CGRP family, is the most likely endogenous peptide mediating these effects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 00:18:47