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Titolo:
Effect of ionizing radiation on thymidine uptake, differentiation, and VEGFR2 receptor expression in endothelial cells: The role of VEGF(165)
Autore:
Kermani, P; Leclerc, G; Martel, R; Fareh, J;
Indirizzi:
Ctr Hosp Univ Montreal, Louis Charles Simard Res Ctr, Mol Cardiol Lab, Montreal, PQ H2L 4M1, Canada Ctr Hosp Univ Montreal Montreal PQ Canada H2L 4M1 eal, PQ H2L 4M1, Canada
Titolo Testata:
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
fascicolo: 1, volume: 50, anno: 2001,
pagine: 213 - 220
SICI:
0360-3016(20010501)50:1<213:EOIROT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR-PERMEABILITY FACTOR; FIBROBLAST GROWTH-FACTOR; SMOOTH-MUSCLE CELLS; GENE-EXPRESSION; TYROSINE KINASE; UP-REGULATION; IN-VITRO; ANGIOGENESIS; HYPOXIA; KDR;
Keywords:
beta irradiation; VEGFR2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Leclerc, G Ctr Hosp Univ Montreal, Louis Charles Simard Res Ctr, Mol Cardiol Lab, 1560 Sherbrooke E, Montreal, PQ H2L 4M1, Canada Ctr Hosp Univ Montreal 1560 Sherbrooke E Montreal PQ Canada H2L 4M1
Citazione:
P. Kermani et al., "Effect of ionizing radiation on thymidine uptake, differentiation, and VEGFR2 receptor expression in endothelial cells: The role of VEGF(165)", INT J RAD O, 50(1), 2001, pp. 213-220

Abstract

Purpose: Late thrombosis of irradiated vascular segments may be the consequence of endothelial cell (EC) dysfunction after radiation therapy. We investigated the effects of beta ionizing radiation on human EC viability thymidine uptake, and differentiation. Methods and Materials: Endothelial cells were exposed to P-32-labeled DNA oligonucleotides in incremental doses of 2, 6, and 10 Gy. The modulation ofthe VEGFR2 receptor expression after irradiation and the overall potentialradioprotective effect of VEGF(165) on these functions were assayed. Results: A dose-dependent inhibitory effect of beta irradiation on ECs' thymidine uptake and differentiation was observed. EC viability, however, wasnot affected at levels of radiation up to 10 Gy, VEGF(165) proved to have a radioprotective effect as ECs' thymidine uptake, after radiation doses of2, 6, and 10 Gy, was increased by 1.5-, 2-, and 4-fold, respectively, in the presence of 10 ng/ml of VEGF(165) (p < 0.05 vs. LacZ), This concentration of VEGF(165) also proved beneficial in maintaining cell differentiation at 16 h postirradiation when compared to controls. These biologic effects were in direct correlation with the upregulation of VEGFR2 receptor expression in irradiated ECs,Conclusions: <beta> irradiation interacts directly with EC functions by significantly reducing their ability to differentiate and proliferate, associated with upregulation of VEGFR2, These effects can be prevented in part bypretreating cells with VEGF(165), an effect potentially favored by the upregulation of VEGFR2 receptor expression after irradiation, (C) 2001 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 02:35:40