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Titolo:
Handling individual mammalian embryos using microfluidics
Autore:
Glasgow, IK; Zeringue, HC; Beebe, DJ; Choi, SJ; Lyman, JT; Chan, NG; Wheeler, MB;
Indirizzi:
Univ Illinois, Beckman Inst Adv Sci & Technol, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 Adv Sci & Technol, Urbana, IL 61801 USA Univ Illinois, Dept Elect & Comp Engn, Urbana, IL 61801 USA Univ IllinoisUrbana IL USA 61801 Elect & Comp Engn, Urbana, IL 61801 USA Univ Wisconsin, Beckman Inst Adv Sci & Technol, Madison, WI 53706 USA UnivWisconsin Madison WI USA 53706 Sci & Technol, Madison, WI 53706 USA Univ Wisconsin, Dept Mech Engn, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 Dept Mech Engn, Madison, WI 53706 USA
Titolo Testata:
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
fascicolo: 5, volume: 48, anno: 2001,
pagine: 570 - 578
SICI:
0018-9294(200105)48:5<570:HIMEUM>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-CELL DEFORMABILITY; IN-VITRO CULTURE; BOVINE EMBRYOS; MOUSE; REQUIREMENTS; TRANSPORT; SYSTEM; CATTLE;
Keywords:
embryos; manipulation; microfluidics;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Engineering, Computing & Technology
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Beebe, DJ Univ Wisconsin, Dept Biomed Engn, 274 Comp Aided Engn Bldg,1410 Engn Dr, Madison, WI 53706 USA Univ Wisconsin 274 Comp Aided Engn Bldg,1410Engn Dr Madison WI USA 53706
Citazione:
I.K. Glasgow et al., "Handling individual mammalian embryos using microfluidics", IEEE BIOMED, 48(5), 2001, pp. 570-578

Abstract

We have designed, built, and tested microfluidic systems capable of transporting individual, preimplantation mouse embryos (100-mum to 150-mum diameter) through a network of channels. Typical channels are 160 to 200 mum deep, 250 to 400 mum wide at the top, and narrower at the bottom (0 to 250 mum wide) due to the fabrication process, In these channels, a pressure gradient of 1 Pa/mm causes the medium to flow on the order of 10(-10) m(3)/s (100 nl/s), with an average speed of 1 to 2 mm/s, Under these flow conditions the embryos roll along the bottoms of the channels, traveling at 1/2 the speed of the fluid, By manipulating the pressure at the wells connected to the ends of the channels, the embryos can be transported to land retained at) specific locations including culture compartments and retrieval wells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 22:33:40