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Titolo:
Characterization and tumorigenicity of human ovarian surface epithelial cells immortalized by SV40 large T antigen
Autore:
Nitta, M; Katabuchi, H; Ohtake, H; Tashiro, H; Yamaizumi, M; Okamura, H;
Indirizzi:
Kumamoto Univ, Sch Med, Dept Obstet & Gynecol, Kumamoto 8608556, Japan Kumamoto Univ Kumamoto Japan 8608556 & Gynecol, Kumamoto 8608556, Japan Kumamoto Univ, Sch Med, Inst Mol Embryol & Genet, Kumamoto 8608556, Japan Kumamoto Univ Kumamoto Japan 8608556 ol & Genet, Kumamoto 8608556, Japan
Titolo Testata:
GYNECOLOGIC ONCOLOGY
fascicolo: 1, volume: 81, anno: 2001,
pagine: 10 - 17
SICI:
0090-8258(200104)81:1<10:CATOHO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
EUROPEAN CASE-CONTROL; SV40-TRANSFORMED HUMAN; POOLED ANALYSIS; CANCER; SIMIAN-VIRUS-40; MESOTHELIOMAS; GROWTH; TUMORS; TRANSFORMATION; TRANSFECTION;
Keywords:
ovarian surface epithelium; SV40 large T antigen; immortalization; ovarian cancer; human;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Nitta, M Kumamoto Univ, Sch Med, Dept Obstet & Gynecol, Kumamoto 8608556, Japan Kumamoto Univ Kumamoto Japan 8608556 l, Kumamoto 8608556, Japan
Citazione:
M. Nitta et al., "Characterization and tumorigenicity of human ovarian surface epithelial cells immortalized by SV40 large T antigen", GYNECOL ONC, 81(1), 2001, pp. 10-17

Abstract

Objectives. Epithelial ovarian cancers are considered to arise from neoplastic transformation of the ovarian surface epithelium (OSE). However, the earliest events in ovarian carcinogenesis have not been clearly defined because patients are often diagnosed in the advanced stages and useful in vivo and in vitro experimental systems using human OSE cells are lacking. We aimed to improve the availability of experimental models for the study of human ovarian carcinogenesis. Methods. Subcultured human OSE cells were transfected with SV40 large T antigen. Resulting OSE cell lines were characterized using immunocytochemistry and tested tumorigenicity. Results. Six immortalized OSE cell lines were obtained. All cell lines essentially retained the original morphological features of normal OSE cells and showed higher proliferation rates and saturation density. Although they were all nontumorigenic in athymic mice, OSE2b-2 sv cells, which were selected in soft agar from colonies of an SV40 large T antigen-expressing transfectant, OSE2b sv, produced tumors on the peritoneal surface, mesothelium, and diaphragm and induced ascites after being injected intraperitoneally. Solid tumors also grew when mice were inoculated subcutaneously. The tumor cells were formed in a solid sheet arrangement and no evidence of glandular or squamous differentiation was present. They were weakly immunostained withan antibody against cytokeratin, and intercellular junctions resembling attachment devices were ultrastructurally present between cells. The tumors were histologically diagnosed as undifferentiated carcinomas. Conclusions. The established cell lines may provide a model system to investigate the mechanisms of cytogenic and molecular changes from normal OSE cells through the various steps of transformation. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:55:28