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Titolo:
A pyrophosphate bridge links the pyruvate-containing secondary cell wall polymer of Paenibacillus alvei CCM 2051 to muramic acid
Autore:
Schaffer, C; Muller, N; Mandal, PK; Christian, R; Zayni, S; Messner, P;
Indirizzi:
Agr Univ Vienna, Zentrum Ultrastrukturforsch, A-1180 Vienna, Austria Agr Univ Vienna Vienna Austria A-1180 kturforsch, A-1180 Vienna, Austria Agr Univ Vienna, Ludwig Boltzmann Inst Mol Nanotechnol, A-1180 Vienna, Austria Agr Univ Vienna Vienna Austria A-1180 anotechnol, A-1180 Vienna, Austria Sci Software Co, A-1140 Vienna, Austria Sci Software Co Vienna Austria A-1140 oftware Co, A-1140 Vienna, Austria Johannes Kepler Univ, Inst Chem, A-4040 Linz, Austria Johannes Kepler Univ Linz Austria A-4040 Inst Chem, A-4040 Linz, Austria
Titolo Testata:
GLYCOCONJUGATE JOURNAL
fascicolo: 10, volume: 17, anno: 2000,
pagine: 681 - 690
SICI:
0282-0080(200010)17:10<681:APBLTP>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SURFACE-LAYER GLYCOPROTEIN; BACILLUS-STEAROTHERMOPHILUS PV72/P2; PULSED FIELD GRADIENTS; N-TERMINAL PART; THERMOSACCHAROLYTICUM E207-71; LINEAR PREDICTION; GLYCAN CHAIN; S-LAYERS; PEPTIDOGLYCAN; PROTEINS;
Keywords:
Paenibacillus alvei; secondary cell wall polymer (SCWP); surface layer (S-layer); peptidoglycan; NMR spectroscopy; structure determination;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Messner, P Agr Univ Vienna, Zentrum Ultrastrukturforsch, Gregor-Mendel-Str33, A-1180Vienna, Austria Agr Univ Vienna Gregor-Mendel-Str 33 Vienna Austria A-1180 ria
Citazione:
C. Schaffer et al., "A pyrophosphate bridge links the pyruvate-containing secondary cell wall polymer of Paenibacillus alvei CCM 2051 to muramic acid", GLYCOCON J, 17(10), 2000, pp. 681-690

Abstract

The peptidoglycan, the secondary cell wall polymer (SCWP), and the surfacelayer (S-layer) glycoprotein are the major glycosylated cell wall components of Paenibacillus alvei CCM 2051. In this report, the complete structure of the SCWP, its linkage to the peptidoglycan layer, and its physicochemical properties have been investigated. From the combined evidence of chemicaland structural analyses together with one- and two-dimensional nuclear magnetic resonance spectroscopy, the following structure of the SCWP-peptidoglycan complex is proposed:[(Pyr4,6)-beta -D-ManpNAc-(1 -->4)-beta -D-GlcpNAc-(1 -->3)](n similar to 11)-(Pyr4,6)-beta -D-ManpNAc-(1 -->4)-alpha -D-GlcpNAc-(1 -->O)-PO2-O-PO2-(O -->6)-MurNAc-Each disaccharide unit is substituted by 4,6-linked pyruvic acid residues. Under mild acidic conditions, up to 50% of them are lost, leaving non-substituted ManNAc residues. The anionic glycan chains constituting the SCWP are randomly linked via pyrophosphate groups to C-6 of muramic acid residues of the peptidoglycan layer. P-31 NMR reveals two signals that, as a consequence of micelle formation, experience different line broadening. Therefore,their integral ratio deviates significantly from 1:1. By treatment with ethylenediaminetetraacetic acid, sodium dodecyl sulfate, and sonication immediately prior to NMR measurement, this ratio approaches unity. The reversibility of this behavior corroborates the presence of a pyrophosphate linker in this SCWP-peptidoglycan complex. In addition to the determination of the structure and linkage of the SCWP,a possible scenario for its biological function is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 12:51:59