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Titolo:
Regulation of activator/dissociation transposition by replication and DNA methylation
Autore:
Ros, F; Kunze, R;
Indirizzi:
Univ Munich, Inst Mikrobiol & Genet, D-80638 Munich, Germany Univ Munich Munich Germany D-80638 biol & Genet, D-80638 Munich, Germany
Titolo Testata:
GENETICS
fascicolo: 4, volume: 157, anno: 2001,
pagine: 1723 - 1733
SICI:
0016-6731(200104)157:4<1723:ROATBR>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSPOSABLE ELEMENT AC; MAIZE ACTIVATOR TRANSPOSASE; V(D)J RECOMBINATION; SUBTERMINAL SEQUENCES; PUTATIVE TRANSPOSASE; PETUNIA PROTOPLASTS; TRANSIENT ASSAY; BINDING DOMAIN; DS ELEMENT; ZEA-MAYS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Kunze, R Univ Cologne, Bot Inst 2, Gyrhofstr 15, D-50931 Cologne, Germany Univ Cologne Gyrhofstr 15 Cologne Germany D-50931 logne, Germany
Citazione:
F. Ros e R. Kunze, "Regulation of activator/dissociation transposition by replication and DNA methylation", GENETICS, 157(4), 2001, pp. 1723-1733

Abstract

In maize the transposable elements Activator/Dissociation (Ac/Ds) transpose shortly after replication from one of the two resulting chromatids ("chromatid selectivity"). A model has been suggested that explains this phenomenon as a consequence of different affinity for Ac transposase binding to holo-, hemi-, and unmethylated transposon ends. Here we demonstrate that in petunia cells a holomethylated Ds in unable to excise from a nonreplicating vector and that replication restores excision. A Ds element hemi-methylated on one DNA strand transposes in the absence of replication, whereas hemi-methylation of the complementary strand causes a >6.3-fold inhibition of Ds excision. Consistently in the active hemi-methylated state. the Ds ends havea high binding affinity for the transposase, whereas binding to inactive ends is strongly reduced. These results provide strong evidence for tile above-mentioned model. Moreover, in the absence of DNA methylation, replication enhances Ds transposition in petunia protoplasts >8-fold and promotes formation of a predominant excision footprint. Accordingly, replication also has a methylation-independent regulatory effect on transposition.

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Documento generato il 03/12/20 alle ore 21:45:41