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Titolo:
Perinatal changes of I-h in phrenic motoneurons
Autore:
Di Pasquale, E; Tell, F; Ptak, K; Monteau, R; Hilaire, G;
Indirizzi:
Fac Sci St Jerome, CNRS, ESA 6034, F-13397 Marseille 20, France Fac Sci StJerome Marseille France 20 6034, F-13397 Marseille 20, France Northwestern Univ, Sch Med, Dept Physiol, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 ept Physiol, Chicago, IL 60611 USA CNRS, UPR 9011, F-13402 Marseille 20, France CNRS Marseille France 20CNRS, UPR 9011, F-13402 Marseille 20, France
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 7, volume: 13, anno: 2001,
pagine: 1403 - 1410
SICI:
0953-816X(200104)13:7<1403:PCOIIP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYPERPOLARIZATION-ACTIVATED CURRENT; XENOPUS SPINAL NEURONS; INWARD RECTIFICATION; CATION CURRENT; BRAIN-STEM; DEVELOPMENTAL-CHANGES; RESPIRATORY ACTIVITY; PYRAMIDAL NEURONS; FETAL-RAT; PACEMAKER;
Keywords:
foetal and newborn rat; in vitro; maturation; respiration; spinal cord;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Di Pasquale, E Fac Sci St Jerome, CNRS, ESA 6034, F-13397 Marseille 20, France Fac Sci St Jerome Marseille France 20 arseille 20, France
Citazione:
E. Di Pasquale et al., "Perinatal changes of I-h in phrenic motoneurons", EUR J NEURO, 13(7), 2001, pp. 1403-1410

Abstract

The hyperpolarization-activated cationic current (I-h) was characterized and its maturation studied on phrenic motoneurons (PMNs), from reduced preparations of foetal (E18 and E21) and newborn (P0-P3) rats, using the whole-cell patch-clamp technique. In voltage-clamp mode, 2-s hyperpolarizing steps(5-mV, -50 to -110 mV) elicited a noninactivating inward current, blocked by external application of Cs+ or ZD 7283. At -110 mV, I-h current density averaged 0.67 +/- 0.41 pA/pF at E18, reached a transient peak at E21 (1.38 /- 0.11 pA/pF) and decreased at P0-P3 (0.77 +/- 0.22 pA/pF). V-1/2 was similar at E18 and E21 (-79 mV) but was significantly hyperpolarized at P0-P3 (-90 mV). The time constant of activation was voltage-dependent, and significantly faster at E21. Reversal potential was similar at all ages when estimated by extrapolation or tail current procedures. It was positively shifted by 25 +/- 6 mV when external potassium was raised from 3 to 10 mM, suggesting a similar sensitivity to K+ from E18 to P0-3. Cs+ or ZD 7288 applications on PMNs at rest in current-clamp mode, in a partitioned chamber, induced a 10 +/- 2 mV hyperpolarization at E18 and E21, and an 8 +/- 2 mV hyperpolarization at P0-3. The area of the central respiratory drive potential or current was increased by 33 and 31%, respectively, at E21, but was not significantly modified at E18 and P0-3. Our data suggest a critical period during the perinatal maturation of I-h during which it is transiently upregulated and attenuates the influence of the central respiratory drive on PMNs just prior to birth.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:15:41