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Titolo:
Retrograde response of the rat facial motor nucleus to muscle inflammationelicited by phytohaemagglutinin
Autore:
Mariotti, R; Tongiorgi, E; Bressan, C; Armellin, M; Kristensson, K; Bentivoglio, M;
Indirizzi:
Fac Med, Sect Anat & Histol, Dept Morphol & Biomed Sci, I-37134 Verona, Italy Fac Med Verona Italy I-37134 Morphol & Biomed Sci, I-37134 Verona, Italy Univ Trieste, Dept Biol, BRAIN Ctr Neurosci, I-34127 Trieste, Italy Univ Trieste Trieste Italy I-34127 Ctr Neurosci, I-34127 Trieste, Italy Karolinska Inst, Dept Neurosci, Stockholm, Sweden Karolinska Inst Stockholm Sweden Inst, Dept Neurosci, Stockholm, Sweden
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 7, volume: 13, anno: 2001,
pagine: 1329 - 1338
SICI:
0953-816X(200104)13:7<1329:RROTRF>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMYOTROPHIC-LATERAL-SCLEROSIS; NITRIC-OXIDE SYNTHASE; NADPH-DIAPHORASE ACTIVITY; MONOCLONAL-ANTIBODIES; TRACING METHOD; SPINAL-CORD; CELL-DEATH; PHA-L; BCL-2; BRAIN;
Keywords:
Bcl-2; microglia; motoneurons; nitric oxide synthase; T-cell mitogens;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Bentivoglio, M Fac Med, Sect Anat & Histol, Dept Morphol & Biomed Sci, Strada Le Grazie 8, I-37134 Verona, Italy Fac Med Strada Le Grazie 8 Verona Italy I-37134 ona, Italy
Citazione:
R. Mariotti et al., "Retrograde response of the rat facial motor nucleus to muscle inflammationelicited by phytohaemagglutinin", EUR J NEURO, 13(7), 2001, pp. 1329-1338

Abstract

To investigate whether motoneurons react to signals deriving from target inflammation, we studied the facial motor nucleus after injections of phytohaemagglutinin in the snout of adult rats. This plant lectin is a tool widely used to induce proliferation and activation of T lymphocytes, and we observed marked lymphocyte infiltration in the injected facial muscles. Retrograde labelling of motoneurons was not detected after peripheral injections of fluorochrome-conjugated phytohaemagglutinin. Nitric oxide synthase, revealed by NADPH-diaphorase histochemistry, OX-42-immunoreactive microglia, andexpression of the cell death repressor gene bcl-2, investigated with nonradioactive in situ hybridization and immunohistochemistry, were evaluated inthe facial nucleus. Daily phytohaemagglutinin injections for 4 days, mimicking repeated muscle exposure to inflammatory stimuli, resulted after 2-daysurvival in NADPH-diaphorase induction in motoneurons and marked activation of the surrounding microglia. Quantitative image analysis of NADPH-diaphorase staining, and OX-42 immunoreactivity and microglial cell counts indicated highly significant increases with respect to saline-injected control cases. The occurrence of a neuroprotective retrograde response was evaluated monitoring bcl-2 expression. Following single phytohaemagglutinin administration, bcl-2 mRNA was significantly upregulated at 6 h in facial motoneurons and returned to basal levels at 24 h. Bcl-2 immunoreactivity was markedlyupregulated at 24 h and was still significantly higher than in controls at7 days, when concomitant NADPH-diaphorase induction in motoneurons and microglia activation was also observed. No degenerative features were observedin motoneurons after phytohaemagglutinin injections at the examined time-points. The data point out that local muscle inflammation retrogradely elicits gene activation in motoneurons and their microenvironment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 22:25:20