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Titolo:
Ca2+ signalling and changes of mitochondrial function during low-Mg2+-induced epileptiform activity in organotypic hippocampal slice cultures
Autore:
Kovacs, R; Schuchmann, S; Gabriel, S; Kardos, J; Heinemann, U;
Indirizzi:
Hungarian Acad Sci, Chem Res Ctr, H-1025 Budapest, Hungary Hungarian Acad Sci Budapest Hungary H-1025 Ctr, H-1025 Budapest, Hungary Humboldt Univ, Johannes Muller Inst Physiol, Berlin, Germany Humboldt Univ Berlin Germany annes Muller Inst Physiol, Berlin, Germany
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 7, volume: 13, anno: 2001,
pagine: 1311 - 1319
SICI:
0953-816X(200104)13:7<1311:CSACOM>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBRAL BLOOD-FLOW; SUPEROXIDE GENERATION; HEART-MITOCHONDRIA; ENTORHINAL CORTEX; EFFLUX MECHANISM; INDUCED SEIZURES; RAT HIPPOCAMPUS; CELL-DEATH; NEURONS; CALCIUM;
Keywords:
free radicals; hippocampus; organotypic slice cultures; seizure-like events; Mg2+ free; calcium; mitochondria; NADPH; Delta Psi;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Kovacs, R Hungarian Acad Sci, Chem Res Ctr, Pusztaszeri U 59-67, H-1025 Budapest, Hungary Hungarian Acad Sci Pusztaszeri U 59-67 Budapest Hungary H-1025
Citazione:
R. Kovacs et al., "Ca2+ signalling and changes of mitochondrial function during low-Mg2+-induced epileptiform activity in organotypic hippocampal slice cultures", EUR J NEURO, 13(7), 2001, pp. 1311-1319

Abstract

Several lines of evidence indicate that augmented neuronal activity is associated with increased mitochondrial function, however, the mechanisms of coupling are still unclear. In this study we used a low extracellular Mg2+ concentration and short stimulus trains to evoke neuronal hyperactivity in the form of seizure-like events (SLE) in hippocampal slice cultures. Simultaneous microfluorimetric and electrophysiological techniques were applied togain insight into changes of Ca2+ concentration in different compartments and into mitochondrial function. SLEs were associated with a large decreaseof the extracellular Ca2+ concentration ([Ca2+]e), a spiking increase of the cytoplasmic and a smoothed elevation of the mitochondrial Ca2+ concentration (cytoplasmic concentration [Ca2+]i; intramitrochondrial concentration [Ca2+]m). Following an initial apparent decline in the mitochondrial membrane potential (Delta Psi) and NAD(P)H autofluorescence, mitochondria depolarized and NADH production was augmented. Furthermore, SLEs were associated with increased oxidation of dihydroethidine (HEt). Our data Suggest that intramitochondrial Ca2+ accumulation stimulates NADH production and productionof radical oxygen species (ROS). Interestingly, mitochondrial depolarization followed [Ca2+]i and [Ca2+]m changes with a delay implying that electrogenic extrusion of Ca2+ from the mitochondrial matrix might be responsible for the depolarization of the mitochondrial membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 19:47:23