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Titolo:
Phorbol esters promote postsynaptic accumulation of Vesl-1S/Homer-1a protein
Autore:
Kato, A; Fukuda, T; Fukazawa, Y; Isojima, Y; Fujitani, K; Inokuchi, K; Sugiyama, H;
Indirizzi:
Kyushu Univ, Grad Sch Sci, Dept Biol, Higashi Ku, Fukuoka 8128581, Japan Kyushu Univ Fukuoka Japan 8128581 ol, Higashi Ku, Fukuoka 8128581, Japan Kyushu Univ, Grad Sch Med Sci, Dept Anat & Neurobiol, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ol, Higashi Ku, Fukuoka 8128582, Japan Mitsubishi Kasei Inst Life Sci, Machida, Tokyo 1948511, Japan Mitsubishi Kasei Inst Life Sci Machida Tokyo Japan 1948511 1948511, Japan Osaka Univ, Inst Prot Res, Div Prot Metab, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 t Metab, Suita, Osaka 5650871, Japan
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 7, volume: 13, anno: 2001,
pagine: 1292 - 1302
SICI:
0953-816X(200104)13:7<1292:PEPPAO>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
METABOTROPIC GLUTAMATE RECEPTORS; GTPASE-ACTIVATING PROTEIN; LONG-TERM POTENTIATION; KINASE-C; RAT-BRAIN; N-CHIMAERIN; IMMUNOCYTOCHEMICAL LOCALIZATION; SYNAPTIC TRANSMISSION; HIPPOCAMPAL-NEURONS; DENSITY PROTEINS;
Keywords:
immunocytochemistry; rat; subcellular distribution; vesl-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Kato, A Kyushu Univ, Grad Sch Sci, Dept Biol, Higashi Ku, Fukuoka 8128581,Japan Kyushu Univ Fukuoka Japan 8128581 shi Ku, Fukuoka 8128581, Japan
Citazione:
A. Kato et al., "Phorbol esters promote postsynaptic accumulation of Vesl-1S/Homer-1a protein", EUR J NEURO, 13(7), 2001, pp. 1292-1302

Abstract

We examined effects of phorbol esters on the amount and the subcellular distribution of the activity-regulated protein Vesl-1S/ Homer-la in cultured hippocampal neurons. Major Vesl-1S immunoreactivity (IR) was detected throughout neuronal somata under control conditions. Bath application of phorbolesters, PMA and PDBU resulted in the increase in the amount of Vesl-1S proteins and promoted punctate distribution of Vesl-1S IR at the cortical regions of the neuronal somata. Immunofluorescent observations using antisynaptophysin and anti-Vesl-1S antibodies, and electron microscopic observations,revealed that Vesl-1S accumulated at postsynaptic regions following PMA application. Membrane depolarization with high concentrations of external potassium also promoted the punctate distribution of Vesl-1S IR. These resultsdemonstrate that phorbol-triggered reaction cascades result in the accumulation of Ves-1S protein at postsynaptic regions, and suggest that these phorbol effects may mimic those caused by synaptic activities.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 22:19:51