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Titolo:
Recognition of basic fuchsin prestained microfissures of intravital originwith fluorescence microscopy: validation of a shortcut
Autore:
Frisch, T; Sorensen, MS; Bretlau, P;
Indirizzi:
Rigshosp, Dept Otolaryngol Head & Neck Surg, Otopathol Lab, DK-2100 Copenhagen O, Denmark Rigshosp Copenhagen Denmark O opathol Lab, DK-2100 Copenhagen O, Denmark
Titolo Testata:
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
fascicolo: 2, volume: 258, anno: 2001,
pagine: 55 - 60
SICI:
0937-4477(200102)258:2<55:ROBFPM>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANCELLOUS BONE; TEMPORAL BONE; FAILURE;
Keywords:
prestaining; bulk-staining; microfissures; microdamage; artifacts; undecalcified; perilabyrinthine bone; otic capsule; fluorescence microscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Frisch, T Rigshosp, Dept Otolaryngol Head & Neck Surg, Otopathol Lab, F-2071,Blegdamsvej 9, DK-2100 Copenhagen O, Denmark Rigshosp F-2071,Blegdamsvej9 Copenhagen Denmark O n O, Denmark
Citazione:
T. Frisch et al., "Recognition of basic fuchsin prestained microfissures of intravital originwith fluorescence microscopy: validation of a shortcut", EUR ARCH OT, 258(2), 2001, pp. 55-60

Abstract

For 70 years it has been suspected that not all microfissures in histological bone sections are artifacts, but that some are provoked in vivo throughrepetitive stress. The development of undecalcified bone techniques and ofthe bulk staining technique has established a method for demonstrating theexistence of intravital cracks and enhanced the discrimination towards artifactual microfissures in the load-bearing skeleton. Recently the presence of intravital microfissures has also been ascertained in temporal bones by these techniques. Due to the fluorescent properties of basic fuchsin it is possible to use epifluorescence microscopy for analysis of microfissures after bulk staining with basic fuchsin. This provides a more steady microscopic background and a sharper delineation of surface level structures since no projection from lower levels interfere. Artifactual cracks, which in transmitted light microscopy may look like darkly stained intravital microfissures due to refraction phenomena, be come invisible or colorless. Epifluorescence microscopy enhances the detection of both smaller and larger pre-stained intravital microfissures, and leaves only a minor part of the cracks without certain categorization. The epifluorescence mode of analysis has the further advantage of being independent of slice thickness, making feasible whole-specimen analysis by serial stepwise grinding. The present study shows that the number and the length of microfissures in the human otic capsule, counted and measured under the epifluorescence microscope, equals numerically the findings in light microscopy enabling the routine use of this modeof analysis. This may prove to be of particular value in the research intothe etiology and pathogenesis of otosclerosis as well as perilymphatic fistulae.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 14:26:44