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Titolo:
A highly efficient ligand-regulated Cre recombinase mouse line shows that LoxP recombination is position dependent
Autore:
Vooijs, M; Jonkers, J; Berns, A;
Indirizzi:
Netherlands Canc Inst, Div Mol Genet, NL-1066 CX Amsterdam, Netherlands Netherlands Canc Inst Amsterdam Netherlands NL-1066 CX rdam, Netherlands Netherlands Canc Inst, Ctr Biomed Genet, NL-1066 CX Amsterdam, NetherlandsNetherlands Canc Inst Amsterdam Netherlands NL-1066 CX rdam, Netherlands
Titolo Testata:
EMBO REPORTS
fascicolo: 4, volume: 2, anno: 2001,
pagine: 292 - 297
SICI:
1469-221X(200104)2:4<292:AHELCR>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
SITE-SPECIFIC RECOMBINATION; DNA RECOMBINATION; SOMATIC MUTAGENESIS; TRANSGENIC MICE; MAMMALIAN-CELLS; MUTANT MICE; STEM-CELLS; GENE; EXPRESSION; INACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Berns, A Netherlands Canc Inst, Div Mol Genet, Plesmanlaan 121, NL-1066 CXAmsterdam, Netherlands Netherlands Canc Inst Plesmanlaan 121 Amsterdam Netherlands NL-1066 CX
Citazione:
M. Vooijs et al., "A highly efficient ligand-regulated Cre recombinase mouse line shows that LoxP recombination is position dependent", EMBO REP, 2(4), 2001, pp. 292-297

Abstract

Conditional gene inactivation using the Cre/loxP system is widely used, but the difficulty in properly regulating Cre expression remains one of the bottlenecks. One approach to regulate Cre activity utilizes a mutant estrogen hormone-binding domain (ERT) to keep Cre inactive unless the nonsteroidalestrogen analog 4-hydroxytamoxifen (OHT) is present. Here we describe a mouse strain expressing Cre-ERT from the ubiquitously expressed ROSA26 (R26) locus. We demonstrate efficient temporal and spatial regulation of Cre recombination in vivo and in primary cells derived from these mice. We show theexistence of marked differences in recombination frequencies between different substrates within the same cell. This has important consequences when concurrent switching of multiple alleles within the same cell is needed, and highlights one of the difficulties that may be encountered when using reporter mice as indicator strains.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:23:21