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Titolo:
The B cell superantigen-like interaction of intravenous immunoglobin (IVIG) with Fab fragments of V-H 3-23 and 3-30/3-30.5 germline gene origin cloned from a patient with Kawasaki disease is enhanced after IVIG therapy
Autore:
Leucht, S; Uttenreuther-Fischer, MM; Gaedicke, G; Fischer, P;
Indirizzi:
Humboldt Univ, Charite,Otto Heubner Ctr, Childrens Hosp, Mol Biol Lab, D-10117 Berlin, Germany Humboldt Univ Berlin Germany D-10117 l Biol Lab, D-10117 Berlin, Germany
Titolo Testata:
CLINICAL IMMUNOLOGY
fascicolo: 1, volume: 99, anno: 2001,
pagine: 18 - 29
SICI:
1521-6616(200104)99:1<18:TBCSIO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
IDIOPATHIC THROMBOCYTOPENIC PURPURA; PHAGE DISPLAY LIBRARIES; AUTOIMMUNE THROMBOCYTOPENIA; STRUCTURAL BASIS; BINDING-SITE; ANTIBODIES; REGION; IGG; SURFACE; REPERTOIRE;
Keywords:
antiidiotype; B cell; complementarity-determining region; immunoglobulin; phage display; superantigen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Leucht, S Humboldt Univ, Charite,Otto Heubner Ctr, Childrens Hosp, Mol Biol Lab, D-10117 Berlin, Germany Humboldt Univ Berlin Germany D-10117 , D-10117 Berlin, Germany
Citazione:
S. Leucht et al., "The B cell superantigen-like interaction of intravenous immunoglobin (IVIG) with Fab fragments of V-H 3-23 and 3-30/3-30.5 germline gene origin cloned from a patient with Kawasaki disease is enhanced after IVIG therapy", CLIN IMMUNO, 99(1), 2001, pp. 18-29

Abstract

The etiology of Kawasaki disease (KD) is still unknown. Therefore, the diagnosis relies on clinical criteria only. Although a specific therapy for KDis not available, coronary complications can be significantly reduced withthe help of intravenous immunoglobulin (IVIG) therapy. It is not clear howMG interacts with the immune system. Previously, we selected a large number of IgG and IgM Fab fragments specifically reacting with IVIG molecules byphage display and antiidiotypic panning from three patients with autoimmune thrombocytopenia, a patient with lupus, and a healthy individual. Sequencing revealed that the favored V-H germline gene segments of these MG-bound Fabs were 3-23 or 3-30/3-30.5, the most frequently rearranged V-H genes among human B cells. The binding pattern suggested a B cell superantigen-like,specific interaction of an IVIG subset with B cells that present B cell receptors derives from these two germline genes. The aim of the current studywas to investigate whether treatment with MG influences this restricted interaction. Therefore we cloned and selected Fab fragments from a patient with KD before and after MG therapy. A favored selection of antibodies derived from both the 3-23 and the 3-30/3-30.5 germline gene segments as before was observed. importantly, the reactivity with IVIG was significantly higherfor clones from the library prepared after the IVIG treatment, providing the first in vivo functional evidence that a subset of IVIG may selectively activate B cells of this germline origin. This mechanism may add to the therapeutic effect of IVIG; in the treatment of KD. (C) 2001 Academic Press.

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Documento generato il 01/04/20 alle ore 09:50:39