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Titolo:
Adenovirus-mediated antisense urokinase-type plasminogen activator receptor gene transfer reduces tumor cell invasion and metastasis in non-small cell lung cancer cell lines
Autore:
Lakka, SS; Rajagopal, R; Rajan, MK; Mohan, PM; Adachi, Y; Dink, DH; Olivero, WC; Gujrati, M; Ali-Osman, F; Roth, JA; Yung, WKA; Kyritsis, AP; Rao, JS;
Indirizzi:
UIC Coll Med, Dept Biomed & Therapeut Sci, Div Canc Biol, Peoria, IL 61656USA UIC Coll Med Peoria IL USA 61656 Sci, Div Canc Biol, Peoria, IL 61656USA UIC Coll Med, Dept Neurosurg, Peoria, IL 61656 USA UIC Coll Med Peoria ILUSA 61656 ed, Dept Neurosurg, Peoria, IL 61656 USA UIC Coll Med, Dept Neuropathol, Peoria, IL 61656 USA UIC Coll Med Peoria IL USA 61656 , Dept Neuropathol, Peoria, IL 61656 USA Univ Texas, MD Anderson Canc Ctr, Dept Neurosurg, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 tr, Dept Neurosurg, Houston, TX 77030 USA Univ Texas, MD Anderson Canc Ctr, Dept Thorac & Cardiovasc Surg, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 & Cardiovasc Surg, Houston, TX 77030 USA Univ Texas, MD Anderson Canc Ctr, Dept Cardiovasc Surg, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 pt Cardiovasc Surg, Houston, TX 77030 USA Univ Texas, MD Anderson Canc Ctr, Dept Neurooncol, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 r, Dept Neurooncol, Houston, TX 77030 USA
Titolo Testata:
CLINICAL CANCER RESEARCH
fascicolo: 4, volume: 7, anno: 2001,
pagine: 1087 - 1093
SICI:
1078-0432(200104)7:4<1087:AAUPAR>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-TRANSDUCTION; BREAST-CANCER; GROWTH-FACTOR; IN-VIVO; INHIBITION; EXPRESSION; SYSTEM; PROTEOLYSIS; MECHANISM; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Rao, JS UIC Coll Med, Dept Biomed & Therapeut Sci, Div Canc Biol, 1 IlliniDr, Peoria, IL 61656 USA UIC Coll Med 1 Illini Dr Peoria IL USA 61656 Peoria, IL 61656 USA
Citazione:
S.S. Lakka et al., "Adenovirus-mediated antisense urokinase-type plasminogen activator receptor gene transfer reduces tumor cell invasion and metastasis in non-small cell lung cancer cell lines", CLIN CANC R, 7(4), 2001, pp. 1087-1093

Abstract

The urokinase-type plasminogen activator (uPA) and its receptor (uPAR) play an important role in the proteolytic cascade involved in the metastasis of lung and other cancers. We report that the reduction in uPAR levels produced by an antisense strategy using an adenovirus construct (Ad-uPAR) in H1299 cells, an invasive human lung cancer cell line that produces high levelsof uPAR, resulted in a decrease of uPAR levels to 80-90% of those seen in cells infected with mock or adenovirus (Ad)-cytomegalovirus vector controls. In addition, increasing the multiplicity of infection from 25 to 200 caused a corresponding decrease in the level of uPAR protein within 5 days of treatment, as shown by Western blot analysis. Furthermore, the in vitro translation of total RNA levels of Ad-uPAR-infected H1299 cells in a rabbit reticulocyte lysate system caused a 50-70% decrease in uPAR inmunoprecipitate in Ad-uPAR-infected cells relative to the levels in cells of mock and vector controls. The Matrigel invasion assay showed the invasion of H1299 cells and A549 cells infected with Ad-uPAR to be decreased by 70% relative to mock- and vector-infected controls. Infection of tumor cells with Ad-uPAR before implantation significantly reduced the incidence of lung metastasis by 85% as compared with the control virus-infected cells injected into nude mice through the tail vein. Our collective results show that the uPAR system is a potential target of treatment for lung cancers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 22:27:08