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Titolo:
Femtosecond studies of protein - DNA binding and dynamics: Histone I
Autore:
Zhong, DP; Pal, SK; Zewail, AH;
Indirizzi:
CALTECH, Arthur Amos Noyes Lab Chem Phys, Lab Mol Sci, Pasadena, CA 91125 USA CALTECH Pasadena CA USA 91125 m Phys, Lab Mol Sci, Pasadena, CA 91125 USA
Titolo Testata:
CHEMPHYSCHEM
fascicolo: 4, volume: 2, anno: 2001,
pagine: 219 - 227
SICI:
1439-4235(20010417)2:4<219:FSOP-D>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLVATION DYNAMICS; ELECTRON-TRANSFER; PHOTON-ECHO; FLUORESCENCE; WATER; SPECTROSCOPY; CHROMATIN; POLARITY; SOLVENTS; MECHANISM;
Keywords:
femtosecond dynamics; molecular recognition; protein-DNA interactions;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Zhong, DP CALTECH, Arthur Amos Noyes Lab Chem Phys, Lab Mol Sci, Pasadena,CA 91125 USA CALTECH Pasadena CA USA 91125 b Mol Sci, Pasadena, CA 91125 USA
Citazione:
D.P. Zhong et al., "Femtosecond studies of protein - DNA binding and dynamics: Histone I", CHEMPHYSCHE, 2(4), 2001, pp. 219-227

Abstract

in this contribution, we report studies of the nature of binding interactions and dynamics of protein histone 1 (H1) with ligands in solution and as a complex with DNA, an important biological process for the higher-order structure in chromatin. With femtosecond time resolution, we examined the role of solvation by water, the micropolarity at the interface of the binding site(s) of H1, and the rigidity of the complex structure. We used two biologically common fluorescent probes: 2-(p-toluidino)naphthalene-6-sulfonate (TNS) and 5-(dimethylamino)naphthalene-1-sulfonyl chloride (DC). By noncovalently attaching TNS and covalently adducting DC to the binding sites we found that the solvation dynamics, which occur within 1 ps, for the probe at the protein surface and in bulk solution are comparable, indicating the significant contribution of bulk water shells. However, the local polarity changes significantly reflecting the change in dielectric properties at the protein/water interface. The binding structure of the protein - DNA complex was examined by the local orientational motion of the probe. The covalently bound DC molecule, sandwiched between the protein and DNA, was found to be frozen, revealing the very rigid structure at the recognition site, while, for noncovalently bound TNS, the complexes displace the probe. The dynamicalrigidity of the complex, and the role of solvation and interface polarity,elucidate the strong recognition mechanism between DNA and the protein by electrostatic interactions, which are important to the compactness and to chromatin condensation in the biological function.

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Documento generato il 14/07/20 alle ore 22:51:49