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Titolo:
Junctional proteins and Ca2+ transport in the rat odontoblast-like cell line MRPC-1
Autore:
Lundgren, T; Nilsson, M; Ritchie, HH; Linde, A;
Indirizzi:
Gothenburg Univ, Dept Oral Biochem, Gothenburg, Sweden Gothenburg Univ Gothenburg Sweden Dept Oral Biochem, Gothenburg, Sweden Gothenburg Univ, Inst Anat & Cell Biol, Gothenburg, Sweden Gothenburg Univ Gothenburg Sweden Anat & Cell Biol, Gothenburg, Sweden Univ Michigan, Sch Dent, Dept Cariol Restorat Sci & Endodont, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 & Endodont, Ann Arbor, MI 48109 USA
Titolo Testata:
CALCIFIED TISSUE INTERNATIONAL
fascicolo: 3, volume: 68, anno: 2001,
pagine: 192 - 201
SICI:
0171-967X(200103)68:3<192:JPACTI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
THYROID EPITHELIAL INTEGRITY; N-CADHERIN EXPRESSION; FREEZE-FRACTURE; PLASMA-MEMBRANE; INTERCELLULAR-JUNCTIONS; CALCIUM-TRANSPORT; IODIDE TRANSPORT; GROWTH-FACTOR; TOOTH GERMS; P-CADHERIN;
Keywords:
calcification; dentinogenesis; cadherins; catenins; intercellular junctions;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Lundgren, T Gothenburg Univ, Dept Oral Biochem, Gothenburg, Sweden Gothenburg Univ Gothenburg Sweden ochem, Gothenburg, Sweden
Citazione:
T. Lundgren et al., "Junctional proteins and Ca2+ transport in the rat odontoblast-like cell line MRPC-1", CALCIF TIS, 68(3), 2001, pp. 192-201

Abstract

A transcellular bulk flow of Ca2+ ions through the odontoblast layer is ofcentral importance during dentinogenesis. For this, specialized mechanismsmay exist, which by a concerted action, gate Ca2+ into the proximal end ofthe cells and extrude the ions towards the mineralization front. To elucidate these mechanisms, an in vitro model would be useful. Mature odontoblasts are, however, post-mitotic cells and cannot be propagated in cell culture. The aim of the present study was, therefore, to characterize the odontoblast-like rat cell line MRPC-1(1) with regard to transcellular Ca2+ transport, barrier function, and intercellular junctions when cultured on membranesin Transwell chambers. The MRPC-1 cells grew as epithelial-like cells in acontinuous bilayer separated by a thin collagenous matrix and with intercellular junctional complexes. They exhibited properties of a low-resistance epithelium, maintained a Ca2+-dependent diffusion barrier, and exhibited a functional diversity between the two cell layers. MRPC-1 cells expressed ZO-1. occludin, E-l and N-cadherins in addition to alpha-, beta-, gamma- and p120(cat) catenins, thereby demonstrating some traits in common with, but also differences from. epithelial cells and major differences from fibroblasts. The transcellular Ca2+ flux was inhibitable by nifedipine unidirectionally, giving evidence for an active intracellular Ca2+ transport through voltage-gated channels of the L-type. Similarities with native odontoblasts indicate that MRPC-1 cells may be useful for in vitro studies of transcellular Ca2+ transport mechanisms of importance for the calcification process.

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Documento generato il 29/09/20 alle ore 23:51:47