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Titolo:
Electrical stimulation alters protein adsorption and nerve cell interactions with electrically conducting biomaterials
Autore:
Kotwal, A; Schmidt, CE;
Indirizzi:
Univ Texas, Dept Chem Engn, Austin, TX 78712 USA Univ Texas Austin TX USA78712 exas, Dept Chem Engn, Austin, TX 78712 USA
Titolo Testata:
BIOMATERIALS
fascicolo: 10, volume: 22, anno: 2001,
pagine: 1055 - 1064
SICI:
0142-9612(200105)22:10<1055:ESAPAA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SCIATIC-NERVE; FIBRONECTIN; REGENERATION; GROWTH; OUTGROWTH; POLYMER; VITRONECTIN; CULTURE; INVITRO; FIELDS;
Keywords:
tissue engineering; peripheral nerve regeneration; polypyrrole; electrical stimulation; protein adsorption; fibronectin; PC-12 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Schmidt, CE Univ Texas, Dept Chem Engn, 26th & Speedway,MC C0400, Austin, TX 78712 USA Univ Texas 26th & Speedway,MC C0400 Austin TX USA 78712 12 USA
Citazione:
A. Kotwal e C.E. Schmidt, "Electrical stimulation alters protein adsorption and nerve cell interactions with electrically conducting biomaterials", BIOMATERIAL, 22(10), 2001, pp. 1055-1064

Abstract

Electrical charges have been shown to enhance nerve regeneration; however,the mechanisms for this effect are unclear. One hypothesis is that an electrical stimulus alters the local electrical fields of extracellular matrix molecules, changing protein adsorption. We have investigated this hypothesis - that electrical stimulation increases the adsorption of serum proteins,specifically fibronectin (FN), to the electrically conducting polymer polypyrrole (PP), thereby, increasing neurite extension. PP was used because electrical stimulation of PP has been shown to significantly enhance neurite outgrowth, and more importantly, PP can be formed into conduits to guide nerve regeneration ill vivo. Here, we looked at the effects of electrical stimulation on protein adsorption when an electrical current was applied to PP(1)during protein adsorption (immediate stimulation) and (2) several hoursafter protein adsorption (delayed stimulation). We found that immediate stimulation of PP increases FN adsorption from purified FN and serum-containing solutions. Correspondingly, PC-12 cells grown on PP films that had been previously adsorbed with FN during immediate stimulation expressed longer neurites. However, for delayed stimulation, no significant differences in adsorption or neurite outgrowth were observed. These studies suggest that increased FN adsorption with immediate electrical stimulation may explain enhanced neurite extension on electrically stimulated PP. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 10:33:36