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Titolo:
Propynylated phosphodiester oligonucleotides inhibit ICAM-1 expression in A549 cells on electroporation
Autore:
Meunier, L; Monsigny, M; Roche, AC;
Indirizzi:
CNRS, Ctr Biophys Mol, F-45071 Orleans 02, France CNRS Orleans France 02CNRS, Ctr Biophys Mol, F-45071 Orleans 02, France
Titolo Testata:
ANTISENSE & NUCLEIC ACID DRUG DEVELOPMENT
fascicolo: 2, volume: 11, anno: 2001,
pagine: 117 - 123
SICI:
1087-2906(200104)11:2<117:PPOIIE>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-5 PROPYNE OLIGONUCLEOTIDES; ANTISENSE OLIGODEOXYNUCLEOTIDES; PHOSPHOROTHIOATE ANTISENSE; NUCLEAR DELIVERY; CATIONIC LIPIDS; GENE INHIBITION; PROGRESS; PEPTIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Roche, AC St Jude Childrens Hosp, Dept Tumor Cell Biol, Memphis, TN 38105 USA St Jude Childrens Hosp Memphis TN USA 38105 mphis, TN 38105 USA
Citazione:
L. Meunier et al., "Propynylated phosphodiester oligonucleotides inhibit ICAM-1 expression in A549 cells on electroporation", ANTISENSE N, 11(2), 2001, pp. 117-123

Abstract

Oligodeoxynucleotides (ODN) are used largely as either primers, antisense,or tripler-forming units. Phosphodiester ODN (PO-ODN), which are very rapidly degraded by exonucleases, must be protected at their ends. Even so, their life span inside cells is quite short. Phosphorothioate ODN (PS-ODN) areless sensitive to nucleases and are extensively used as antisense, Unfortunately, unlike PO-ODN, they interact with a number of molecules, including proteins, in addition to their specific nucleic acid targets, Their affinity for their target is lower than that of PO-ODN, PS-ODN containing propyne groups on C5 of pyrimidine have been shown to have a higher affinity towardtheir nucleic acid target. Here, we show that propynylated PO-ODN are morestable and much more efficient than their propyne-free counterparts. They are not efficient when they are used as lipoplexes, but they act as specific antisense on electroporation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:31:16