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Titolo:
HLA-G expression in trophoblast cells circulating in maternal peripheral blood during early pregnancy
Autore:
van Wijk, IJ; Griffioen, S; Tjoa, ML; Mulders, MAM; van Vugt, JMG; Loke, YW; Oudejans, CBM;
Indirizzi:
Free Univ Amsterdam Hosp, Dept Clin Chem, Mol Biol Lab, NL-1007 MB Amsterdam, Netherlands Free Univ Amsterdam Hosp Amsterdam Netherlands NL-1007 MBm, Netherlands Free Univ Amsterdam Hosp, Dept Obstet & Gynecol, NL-1007 MB Amsterdam, Netherlands Free Univ Amsterdam Hosp Amsterdam Netherlands NL-1007 MB m, Netherlands Univ Cambridge, Dept Pathol, Res Grp Human Reprod, Cambridge CB2 1QP, England Univ Cambridge Cambridge England CB2 1QP rod, Cambridge CB2 1QP, England
Titolo Testata:
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
fascicolo: 5, volume: 184, anno: 2001,
pagine: 991 - 997
SICI:
0002-9378(200104)184:5<991:HEITCC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEUKOCYTE ANTIGEN-G; PRENATAL-DIAGNOSIS; FETAL CELLS; PREECLAMPSIA; WOMEN;
Keywords:
aneuploidy; combined immunophenotyping and fluorescence in situ hybridization; HLA-G; noninvasive prenatal diagnosis; trophoblast cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: van Wijk, IJ Free Univ Amsterdam Hosp, Dept Clin Chem, Mol Biol Lab, POB 7057, NL-1007 MB Amsterdam, Netherlands Free Univ Amsterdam Hosp POB 7057 Amsterdam Netherlands NL-1007 MB
Citazione:
I.J. van Wijk et al., "HLA-G expression in trophoblast cells circulating in maternal peripheral blood during early pregnancy", AM J OBST G, 184(5), 2001, pp. 991-997

Abstract

OBJECTIVE: The aim of this study was to assess the use of circulating trophoblast cells in maternal peripheral blood for noninvasive prenatal diagnosis of numeric chromosomal aberrations. STUDY DESIGN: A combined procedure for immunocytochemical identification and deoxyribonucleic acid fluorescence in situ hybridization was used after a single enrichment step consisting of density gradient centrifugation. A specific HLA-G monoclonal antibody was used in combination with X and Y chromosome-specific probes in deoxyribonucleic acid fluorescence in situ hybridization to confirm fetal identity of cells bearing HLA-G in the case of a male fetus. RESULTS: We detected fetal trophoblast cells expressing HLA-G in maternal blood starling at 9 weeks' gestation; In addition to fetal sex prediction with X and Y chromosome-specific probes, fetal aneuploidy was confirmed in peripheral blood from a pregnancy complicated by trisomy 21. CONCLUSION: Although the numbers of fetal cells were extremely low, the proof of concept was demonstrated. Early noninvasive prenatal screening for numeric chromosomal abnormalities with fetal trophoblast cells is feasible.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:49:55