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Titolo:
Overexpression of HUT1 gene stimulates in vivo galactosylation by enhancing UDP-galactose transport activity in Saccharomyces cerevisiae
Autore:
Kainuma, M; Chiba, Y; Takeuchi, M; Jigami, Y;
Indirizzi:
Natl Inst Biosci & Human Technol, Dept Mol Biol, Tsukuba, Ibaraki 3058566,Japan Natl Inst Biosci & Human Technol Tsukuba Ibaraki Japan 3058566 8566,Japan Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanagawa 2360004, Japan KirinBrewery Co Ltd Kanagawa Japan 2360004 nol, Kanagawa 2360004, Japan
Titolo Testata:
YEAST
fascicolo: 6, volume: 18, anno: 2001,
pagine: 533 - 541
SICI:
0749-503X(200104)18:6<533:OOHGSI>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOPLASMIC-RETICULUM; IN-VIVO; YEAST; SUGARS;
Keywords:
galactosylation; S. cerevisiae; HUT1; UDP-galactose transporter-related gene; UDP-galactose transporter; alpha 1,2-galactosyltransferase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Jigami, Y Natl Inst Biosci & Human Technol, Dept Mol Biol, Tsukuba, Ibaraki 3058566,Japan Natl Inst Biosci & Human Technol Tsukuba Ibaraki Japan 3058566
Citazione:
M. Kainuma et al., "Overexpression of HUT1 gene stimulates in vivo galactosylation by enhancing UDP-galactose transport activity in Saccharomyces cerevisiae", YEAST, 18(6), 2001, pp. 533-541

Abstract

Transfer of activated sugar-nucleotides from the cytoplasm to the lumen ofthe Golgi is an essential requirement for glycosylation of glycoproteins, proteoglycans and glycosphingolipids. Although mannosylation is the major modification in the yeast Saccharomyces cerevisiae, several reports suggest the presence of galactose residues on yeast proteins and sphingolipids. We have detected alpha -galactosylated O-linked chitinase by lectin blotting from cells that functionally express the gma12(+) gene, encoding alpha1,2-galactasyltransferase from Schizosaccharomyces pombe. This result implies thepresence of a UDP-galactose transporter in S. cerevisiae. A conserved gene, HUT1, which encodes a putative multi-transmembrane protein, was cloned and characterized for its possible involvement in galactosylation. The HUT1 gene is not essential and is expressed at a relatively low level under the physiological conditions we examined. The disruption of this gene did not show any apparent impairments in glycosylation. However, a temperature- and concentration-dependent increase in UDP-galactose transport activity was detected from cells overexpressing HUT1 in the presence of gma12(+). The surface of these cells was confirmed to carry galactose residues by staining with FITC-conjugated alpha -galactose-specific lectin. These results suggest arole for Hut1p in the transport of UDP-galactose from the cytosol into theGolgi lumen in S. cerevisiae. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:39:44