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Titolo:
Residual oil fly ash inhalation in guinea pigs: Influence of absorbate andglutathione depletion
Autore:
Norwood, J; Ledbetter, AD; Doerfler, DL; Hatch, GE;
Indirizzi:
US EPA, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA US EPA Res Triangle Pk NC USA 27711 & Dev, Res Triangle Pk, NC 27711 USA
Titolo Testata:
TOXICOLOGICAL SCIENCES
fascicolo: 1, volume: 61, anno: 2001,
pagine: 144 - 153
SICI:
1096-6080(200105)61:1<144:ROFAII>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASCORBIC-ACID; CARDIOVASCULAR-DISEASE; HOSPITAL ADMISSIONS; CO-SUPPLEMENTATION; AIR-POLLUTION; HUMAN-PLASMA; LUNG INJURY; VITAMIN-C; ANTIOXIDANT; IRON;
Keywords:
inhalation; nose-only; guinea pigs; bronchoalveolar lavage; nasal lavage; ascorbic acid; uric acid; residual oil fly ash; glutathione particulate matter;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Hatch, GE US EPA, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, MD-82, Res Triangle Pk, NC 27711 USA US EPA MD-82 Res Triangle Pk NC USA 27711angle Pk, NC 27711 USA
Citazione:
J. Norwood et al., "Residual oil fly ash inhalation in guinea pigs: Influence of absorbate andglutathione depletion", TOXICOL SCI, 61(1), 2001, pp. 144-153

Abstract

Inhaled urban particulate matter (PM) often contains metals that appear tocontribute to its toxicity, These particles first make contact with a thinlayer of epithelial lining fluid in the respiratory tract. Antioxidants present in this fluid and in cells might be important susceptibility factors in PM toxicity. We investigated the role of ascorbic acid (C) and glutathione (GSH) as determinants of susceptibility to inhaled residual oil fly ash (ROFA) in guinea pigs (male, Hartley). Guinea pigs mere divided into four groups, +C+GSH, +C-GSH, -C+GSH, and -C-GSH, and exposed to clean air or ROFA(< 2.5 micron diameter, 19-25 mg/m(3) nose-only for 2.0 h). C and/or GSH were lowered by either feeding C-depleted diet (1 <mu>g C/kg diet, 2 weeks) and/or by ip injection of a mixture of buthionine-S,R-sulfosimine (2.7 mmol/kg body weight) and diethylmaleate (1.2 mmol/kg, 2 h prior). Nasal lavage (NL) and bronchoalveolar lavage (BAL) fluid and cells were. examined at 0 hand 24 h postexposure to ROFA, The C-deficient diet lowered C concentrations in BAL fluid and cells and in NL fluid by 90%, and the GSH-depletion regimen lowered both GSH and C in the BAL fluid and cells by 50%. ROFA deposition was calculated at time 0 from lung Ni levels to be 46 mug/g wet lung. In unexposed animals, the combined deficiency of C and GSH modified the cellular composition of cells recovered in lavage fluid, i.e., the increased number of eosinophils and macrophages in BAL fluid. ROFA inhalation increasedlung injury in the -C-GSH group only (evidenced by increased BAL protein, LDH and neutrophils, and decreased BAL macrophages). ROFA exposure decreased C in BAL, and NL at 0 h, and increased BAL C and GSH (2- to 4-fold above normal) at 24 h in nondepleted guinea pigs, but had no effect on C and GSH in depleted guinea pigs, Combined deficiency of C and GSH resulted in the highest macrophage and eosinophil counts of any group. GSH depletion was associated with increased BAL protein and LDH, increased numbers of BAL macrophages and eosinophils, and decreased rectal body temperatures. We conclude that combined deficiency of C and GSH increased susceptibility to inhaled ROFA; caused unusual BAL cellular changes; resulted in lower antioxidant concentrations in BAL than were observed with single deficiencies. Antioxidantdeficiency may explain increased susceptibility to PM in elderly or diseased populations and may have important implications for extrapolating animaltoxicity data to humans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 11:15:19