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Titolo:
Does the autoantibody immunodominant region on thyroid peroxidase include amino acid residues 742-771?
Autore:
Xiong, ZG; Farilla, L; Guo, J; McLachlan, S; Rapoport, B;
Indirizzi:
Cedars Sinai Med Ctr, Cedars Sinai Res Inst, Autoimmune Dis Unit, Los Angeles, CA 90048 USA Cedars Sinai Med Ctr Los Angeles CA USA 90048 , Los Angeles, CA 90048 USA Univ Calif Los Angeles, Sch Med, Los Angeles, CA USA Univ Calif Los Angeles Los Angeles CA USA , Sch Med, Los Angeles, CA USA
Titolo Testata:
THYROID
fascicolo: 3, volume: 11, anno: 2001,
pagine: 227 - 231
SICI:
1050-7256(200103)11:3<227:DTAIRO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
B-CELL EPITOPE; IMMUNOGLOBULIN GENE USAGE; HASHIMOTOS-THYROIDITIS; MOLECULAR-CLONING; MAJOR AUTOANTIGEN; RECOMBINANT; DISEASE; THYROPEROXIDASE; RECOGNITION; ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Rapoport, B Cedars Sinai Med Ctr, Cedars Sinai Res Inst, Autoimmune Dis Unit, 8700 Beverly Blvd,Suite B-131, Los Angeles, CA 90048 USA Cedars Sinai Med Ctr 8700 Beverly Blvd,Suite B-131 Los Angeles CA USA 90048
Citazione:
Z.G. Xiong et al., "Does the autoantibody immunodominant region on thyroid peroxidase include amino acid residues 742-771?", THYROID, 11(3), 2001, pp. 227-231

Abstract

Identification of the thyroid peroxidase (TPO) amino acid residues that comprise the autoantibody immunodominant region is an important goal that hasproven difficult because of the conformational nature of the epitopes involved. Recent data suggest that the immunodominant region has been located. Thus, by autoantibody recognition of tryptic fragments of native TPO, as well as of conformational portions of TPO expressed as cell-free translates, the autoantibody immunodominant region appears to include amino acid residues 742-771, near the C terminus of the ectodomain. To evaluate this deduction, we expressed as cell-free translates the full TPO ectodomain, as well as TPO truncated after residues 741 and 771. The epitopic integrity of thesemolecules was first confirmed by immunoprecipitation by patient sera containing TPO autoantibodies. However, autoantibody recognition could involve aminority of TPO autoantibodies with the individual sera, not fulfilling the strict criteria for immunodominance. In order to obtain definitive data, we performed immunoprecipitations on these TPO variants with four recombinant human monoclonal autoantibodies that define the immunodominant region. All four monoclonal autoantibodies immunoprecipitated TPO 1-741 to the same extent as they did TPO 1-771 and the full TPO ectodomain, indicating that the immunodominant region comprises (at least in large part) amino acid residues upstream of residue 741.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 14:16:11