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Titolo:
Uptake and metabolism of exogenous glycosphingolipids by cultured cells
Autore:
Schwarzmann, G;
Indirizzi:
Univ Bonn, Kekule Inst Organ Chem & Biochem, Gerhard Domagk, D-53121 Bonn,Germany Univ Bonn Bonn Germany D-53121 hem, Gerhard Domagk, D-53121 Bonn,Germany
Titolo Testata:
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
fascicolo: 2, volume: 12, anno: 2001,
pagine: 163 - 171
SICI:
1084-9521(200104)12:2<163:UAMOEG>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTOR; GANGLIOSIDE-MEDIATED MODULATION; BIOTIN-LABELED GANGLIOSIDE; CEREBELLAR GRANULE CELLS; NEURO-BLASTOMA CELLS; GM1 GANGLIOSIDE; IMMUNOELECTRON MICROSCOPY; SKIN FIBROBLASTS; ULTRASTRUCTURAL-LOCALIZATION; TYROSINE PHOSPHORYLATION;
Keywords:
exogenous gangliosides; ganglioside function; biotin-GM1; intralysosomal membranes; semi-truncated glycolipids; ganglioside transport; endocytosis;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
97
Recensione:
Indirizzi per estratti:
Indirizzo: Schwarzmann, G Univ Bonn, Kekule Inst Organ Chem & Biochem, Gerhard Domagk, Gerhard Domagk Str 1, D-53121 Bonn, Germany Univ Bonn Gerhard Domagk Str 1 Bonn Germany D-53121 ermany
Citazione:
G. Schwarzmann, "Uptake and metabolism of exogenous glycosphingolipids by cultured cells", SEM CELL D, 12(2), 2001, pp. 163-171

Abstract

Exogenous glycosphingolipids, especially gangliosides, are used to study transport and metabolism of their endogenous counterparts as well as their role in cell adhesion, cell recognition and signal transduction. Unlike monodispersed solutes, in aqueous media ganglioside molecules aggregate into micelles (or bilayer structures) with a very low critical micellar concentration. Upon addition to cells in culture, exogenous gangliosides bind to the cell surface in three operationally defined modes: loosely associated micelles removable by serum; tightly attached micelles removable by proteases such as trypsin; and ganglioside molecules inserted into the outer leaflet ofthe plasma membrane. As shown by a biotin-labeled derivative of the ganglioside GM1 these inserted molecules are endocytosed and transported to intralysosomal membranes for catabolism. The benefit from using (partially) nondegradable as well as semi-truncated glycosphingolipids in transport studiesis discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 09:33:14