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Titolo:
High-level secretory production of recombinant porcine follicle-stimulating hormone by Pichia pastoris
Autore:
Boze, H; Celine, L; Patrick, C; Fabien, R; Christine, V; Yves, C; Guy, M;
Indirizzi:
ENSAM INRA, UFR Microbiol Ind & Genet Microorganismes, F-34060 Montpellier, France ENSAM INRA Montpellier France F-34060 ismes, F-34060 Montpellier, France INRA, Unite Recepteurs & Commun Cellulares, F-78352 Jouy En Josas, France INRA Jouy En Josas France F-78352 lulares, F-78352 Jouy En Josas, France INRA, CNRS, Unite Physiol Mammiferes Domest 1291, Equipe Gonadotropines, F-37380 Nouzilly, France INRA Nouzilly France F-37380 pe Gonadotropines, F-37380 Nouzilly, France
Titolo Testata:
PROCESS BIOCHEMISTRY
fascicolo: 8-9, volume: 36, anno: 2001,
pagine: 907 - 913
SICI:
1359-5113(200103)36:8-9<907:HSPORP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
METHYLOTROPHIC YEAST; ALCOHOL OXIDASE; EXPRESSION; LYSOZYME; PROTEINS; GENES;
Keywords:
Pichia pastoris; heterologous protein; follicle-stimulating hormone; high cell density cultures; medium composition;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Boze, H ENSAM INRA, UFR Microbiol Ind & Genet Microorganismes, 2 Pl Viala,F-34060Montpellier, France ENSAM INRA 2 Pl Viala Montpellier France F-34060 tpellier, France
Citazione:
H. Boze et al., "High-level secretory production of recombinant porcine follicle-stimulating hormone by Pichia pastoris", PROCESS BIO, 36(8-9), 2001, pp. 907-913

Abstract

An active porcine Follicle-Stimulating Hormone (rFSH) was secreted by a recombinant strain of the methylotrophic yeast Pichia pastoris. The influenceof the carbon source. either methanol or a mixture of methanol with glycerol or sorbitol, on rFSH production was subsequently studied. On a sorbitol/methanol medium using a high cell-density fed-batch culture the rFSH concentration was 93 mg l(-1). The addition, in fed-batch culture, of vitamins and trace elements to the synthetic medium allows optimal biomass and recombinant protein production to be attained. Under these conditions, 187 mg l(-1) of rFSH were produced and the production yield Y-rFSHX reached 2.5 mg rFSH g DCW-1. In continuous culture the carbon source and growth factor supplementation had an identical effect on rFSH synthesis, which reached a maximum concentration of 280 mg l(-1) and a production yield of 3.6 mg rFSH g DCW-1. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 10:57:49