Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Reversible immobilization of lipase on phenylalanine containing hydrogel membranes
Autore:
Arica, MY; Kacar, Y; Ergene, A; Denizli, A;
Indirizzi:
Kirikkale Univ, Dept Biol, TR-71450 Yahsihan Krikkale, Turkey Kirikkale Univ Yahsihan Krikkale Turkey TR-71450 hsihan Krikkale, Turkey Hacettepe Univ, Dept Chem, TR-06532 Beytepe Ankara, Turkey Hacettepe UnivBeytepe Ankara Turkey TR-06532 532 Beytepe Ankara, Turkey
Titolo Testata:
PROCESS BIOCHEMISTRY
fascicolo: 8-9, volume: 36, anno: 2001,
pagine: 847 - 854
SICI:
1359-5113(200103)36:8-9<847:RIOLOP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANDIDA-RUGOSA; POLYMER BEADS; MIEHEI LIPASE; MUCOR-MIEHEI; PURIFICATION; DERIVATIVES; COPOLYMER; SYSTEM; ESTER;
Keywords:
adsorption; immobilization; lipase; phenylalanine; poly(HEMA) membrane;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Arica, MY Kirikkale Univ, Dept Biol, TR-71450 Yahsihan Krikkale, Turkey Kirikkale Univ Yahsihan Krikkale Turkey TR-71450 kkale, Turkey
Citazione:
M.Y. Arica et al., "Reversible immobilization of lipase on phenylalanine containing hydrogel membranes", PROCESS BIO, 36(8-9), 2001, pp. 847-854

Abstract

Poly(2-hydroxyethylmethacrylate-co-methacrylamidophenlyalanine) poly(HEMA-MAPA) membranes were prepared by UV-initiated photopolymerization of HEMA and MAPA. Lipase immobilization onto these membranes from aqueous solutions containing different amounts of lipase at different pH was investigated in a batch system. The lipase adsorption capacity of the membranes was increased as the MAPA ratio increased in the membrane structure. The maximum lipase immobilization capacity of the poly(HEMA-MAPA-3) membrane was 135 mug cm(-2). The optimum temperature was 5 degreesC higher than that of the free enzyme and was significantly broader. The storage stability increased with immobilization. The enzyme could be repeatedly adsorbed and desorbed without any significant loss in adsorption capacity. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 10:19:33