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Titolo:
Sequence haplotypes revealed by sequence-tagged site fine mapping of the Ror1 gene in the centromeric region of barley chromosome 1H
Autore:
Collins, NC; Lahaye, T; Peterhansel, C; Freialdenhoven, A; Corbitt, M; Schulze-Lefert, P;
Indirizzi:
Max Planck Inst Zuchtungsforsch, Biochem Abt, D-50829 Cologne, Germany MaxPlanck Inst Zuchtungsforsch Cologne Germany D-50829 Cologne, Germany John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England John Innes Ctr Plant Sci Res Norwich Norfolk England NR4 7UH olk, England Rhein Westfal TH Aachen, Inst Biol 1, D-52074 Aachen, Germany Rhein Westfal TH Aachen Aachen Germany D-52074 , D-52074 Aachen, Germany
Titolo Testata:
PLANT PHYSIOLOGY
fascicolo: 3, volume: 125, anno: 2001,
pagine: 1236 - 1247
SICI:
0032-0889(200103)125:3<1236:SHRBSS>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
FRAGMENT-LENGTH-POLYMORPHISM; POWDERY MILDEW RESISTANCE; HORDEUM-VULGARE L.; PCR-BASED MARKERS; RUST RESISTANCE; CONFERRING RESISTANCE; RECOMBINATION RATES; DNA POLYMORPHISM; MLO RESISTANCE; CELL-DEATH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Schulze-Lefert, P Max Planck Inst Zuchtungsforsch, Biochem Abt, Carl von Linne Weg 10, D-50829 Cologne, Germany Max Planck Inst Zuchtungsforsch Carl von Linne Weg 10 Cologne Germany D-50829
Citazione:
N.C. Collins et al., "Sequence haplotypes revealed by sequence-tagged site fine mapping of the Ror1 gene in the centromeric region of barley chromosome 1H", PLANT PHYSL, 125(3), 2001, pp. 1236-1247

Abstract

We describe the development of polymerase chain reaction-based, sequence-tagged site (STS) markers for fine mapping of the barley (Hordeum vulgare) Ror1 gene required for broad-spectrum resistance to powdery mildew (Blumeriagraminis f. sp. hordei). After locating Ror1 to the centromeric region of barley chromosome 1H using a combined amplified fragment length polymorphism/restriction fragment-length polymorphism ((RFLP) approach, sequences of RFLP probes from this chromosome region of barley and corresponding genome regions from the related grass species oat (Avena spp.), wheat, and Triticummonococcum were used to develop STS markers. Primers based on the RFLP probe sequences were used to polymerase chain reaction-amplify and directly sequence homologous DNA stretches from each of four parents that were used for mapping. Over 28,000 by from 22 markers Were compared. In addition to oneinsertion/deletion of at least 2.0 kb, 79 small unique sequence polymorphisms were observed, including 65 single nucleotide substitutions, two dinucleotide substitutions, 11 insertion/deletions, and one 5-bp/10-bp exchange. The frequency of polymorphism between any two barley lines ranged from 0.9 to 3.0 kb, and was greatest for comparisons involving an Ethiopian landrace. Haplotype structure was observed in the marker sequences over distances of several hundred basepairs. Polymorphisms in 16 STSs were used to generategenetic markers, scored hv restriction enzyme digestion or by direct sequencing. Over 2,300 segregants from three populations were used in Ror1 linkage analysis, mapping Ror1 to a 0.2- to 0.5-cM marker interval. We discuss the implications of sequence haplotypes and STS markers for the generation of high-density maps in cereals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 20:18:52