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Titolo:
Genetic analysis of amino acid accumulation in opaque-2 maize endosperm
Autore:
Wang, XL; Larkins, BA;
Indirizzi:
Univ Arizona, Dept Plant Sci, Tucson, AZ 85721 USA Univ Arizona Tucson AZUSA 85721 na, Dept Plant Sci, Tucson, AZ 85721 USA
Titolo Testata:
PLANT PHYSIOLOGY
fascicolo: 4, volume: 125, anno: 2001,
pagine: 1766 - 1777
SICI:
0032-0889(200104)125:4<1766:GAOAAA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTIONAL ACTIVATOR OPAQUE-2; INSENSITIVE ASPARTATE KINASE; ZEA-MAYS-L; HOMOSERINE DEHYDROGENASE; NICOTIANA-SYLVESTRIS; TISSUE-CULTURE; LYSINE CONTENT; HIGHER-PLANTS; EXPRESSION; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Larkins, BA Univ Arizona, Dept Plant Sci, Tucson, AZ 85721 USA Univ Arizona Tucson AZ USA 85721 nt Sci, Tucson, AZ 85721 USA
Citazione:
X.L. Wang e B.A. Larkins, "Genetic analysis of amino acid accumulation in opaque-2 maize endosperm", PLANT PHYSL, 125(4), 2001, pp. 1766-1777

Abstract

The opaque-2 mutation in maize (Zea mays) is associated with an increased level of free amino acids (FAA) in the mature endosperm. In particular, there is a high concentration of lysine, the most limiting essential amino acid. To investigate the basis for the high-FAA phenotype of opaque-2 maize, we characterized amino acid accumulation during endosperm development of several wild-type and opaque-2 inbreds. Oh545 omicron2 was found to have an exceptionally high level of FAA, in particular those derived from aspartate (Asp) and intermediates of glycolysis. The FAA content in Oh545 omicron2 is 12 times greater than its wild-type counterpart, and three and 10 times greater than in Oh51A omicron2 and W64A omicron2, respectively. We crossed Oh545 omicron2 to Oh51A omicron2 and analyzed the F-2:3 progeny to identify genetic loci linked with the high FAA level in these mutants. Quantitative trait locus mapping identified four significant loci that account for about 46% of the phenotypic variance. One locus on the long arm of chromosome 2 iscoincident with genes encoding a monofunctional Asp kinase 2 and a bifunctional Asp kinase-homo-Ser dehydrogenase-2, whereas another locus on the short arm of chromosome 3 is linked with a cytosolic triose phosphate isomerase 4. The results suggest an alternation of amino acid and carbon metabolismleads to overproduction and accumulation of FAA in opaque-2 mutants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 21:56:41