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Titolo:
Gibberellin/abscisic acid antagonism in barley aleurone cells: Site of action of the protein kinase PKABA1 in relation to gibberellin signaling molecules
Autore:
Gomez-Cadenas, A; Zentella, R; Walker-Simmons, MK; Ho, THD;
Indirizzi:
Washington Univ, Dept Biol, Plant Biol Program, St Louis, MO 63130 USA Washington Univ St Louis MO USA 63130 iol Program, St Louis, MO 63130 USA Washington State Univ, USDA ARS, Pullman, WA 99164 USA Washington State Univ Pullman WA USA 99164 SDA ARS, Pullman, WA 99164 USA
Titolo Testata:
PLANT CELL
fascicolo: 3, volume: 13, anno: 2001,
pagine: 667 - 679
SICI:
1040-4651(200103)13:3<667:GAAIBA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA-AMYLASE GENE; HORDEUM-VULGARE-L; ABSCISIC-ACID; RESPONSE COMPLEX; HORMONAL-REGULATION; CEREAL ALEURONE; PLASMA-MEMBRANE; MESSENGER-RNA; TRANSDUCTION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Gomez-Cadenas, A Washington Univ, Dept Biol, Plant Biol Program, Campus Box 1137, St Louis,MO 63130 USA Washington Univ Campus Box 1137 St Louis MO USA 63130 USA
Citazione:
A. Gomez-Cadenas et al., "Gibberellin/abscisic acid antagonism in barley aleurone cells: Site of action of the protein kinase PKABA1 in relation to gibberellin signaling molecules", PL CELL, 13(3), 2001, pp. 667-679

Abstract

The antagonism between gibberellins (GA) and abscisic acid (ABA) is an important factor regulating the developmental transition from embryogenesis toseed germination. In barley aleurone layers, the expression of genes encoding alpha -amylases and proteases is induced by GA but suppressed by ABA. It has been shown that an ABA-induced protein kinase, PKABA1, mediates the ABA suppression of alpha -amylase expression. Using a barley aleurone transient expression system, we have now localized the site of action of PKABA1 relative to other signal transduction components governing the expression ofalpha -amylase. The expression of alpha -amylase can be transactivated by the transcription factor GAMyb, which is itself induced by GA. A truncated GAMyb containing the DNA binding domain but lacking the transactivation domain prevents the GA induction of cu-amylase, further supporting the notion that GAMyb mediates the GA induction of alpha -amylase expression. AlthoughABA and PKABA1 strongly inhibit the GA induction of alpha -amylase, they have no effect on GAMyb-transactivated alpha -amylase expression. Using a GAMyb promoter-beta -glucuronidase construct, we also show that both ABA and PKABA1 repress the GA induction of GAMyb. In the slender mutant, GAMyb and alpha -amylase are highly expressed, even in the absence of GA. However, this constitutive expression can still be inhibited by ABA, PKABA1, or an inhibitor of cGMP synthesis. On the basis of these observations, we suggest that PKABA1 acts upstream from the formation of functional GAMyb but downstream from the site of action of the Slender gene product. Because PKABA1 inhibits the GA induction of the GAMyb promoter-beta -glucuronidase construct, it appears that at least part of the action of PKABA1 is to downregulate GAMyb at the transcriptional level.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 02:00:32