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Titolo:
Targeted histone acetylation and altered nuclease accessibility over shortregions of the pea plastocyanin gene
Autore:
Chua, YL; Brown, APC; Gray, JC;
Indirizzi:
Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England Univ CambridgeCambridge England CB2 3EA Sci, Cambridge CB2 3EA, England
Titolo Testata:
PLANT CELL
fascicolo: 3, volume: 13, anno: 2001,
pagine: 599 - 612
SICI:
1040-4651(200103)13:3<599:THAAAN>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIGHT-REGULATED EXPRESSION; TRANSGENIC TOBACCO PLANTS; RNA-POLYMERASE-II; CHROMATIN STRUCTURE; IN-VIVO; ACETYLTRANSFERASE ACTIVITY; ARCHITECTURAL ELEMENTS; HYPERSENSITIVE SITES; ARABIDOPSIS-THALIANA; FUNCTIONAL-ANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
78
Recensione:
Indirizzi per estratti:
Indirizzo: Gray, JC Univ Cambridge, Dept Plant Sci, Downing St, Cambridge CB2 3EA, England Univ Cambridge Downing St Cambridge England CB2 3EA 3EA, England
Citazione:
Y.L. Chua et al., "Targeted histone acetylation and altered nuclease accessibility over shortregions of the pea plastocyanin gene", PL CELL, 13(3), 2001, pp. 599-612

Abstract

The chromatin structure of the pea plastocyanin gene (PetE) was examined at three different transcriptional states by investigating the acetylation states of histones H3 and H4 and the nuclease accessibility of the gene in pea roots, etiolated shoots, and green shoots. The acetylation states of histones associated with different regions of PetE were analyzed by chromatin immunoprecipitation with antibodies specific for acetylated or nonacetylated histone H3 or H4 tails, followed by polymerase chain reaction quantification. Comparison of pea tissues indicated that histone hyperacetylation was associated with increased PetE transcription in green shoots, Moreover, hyperacetylation of both histones H3 and H4 was targeted to the enhancer/promoter region in green shoots, suggesting that only specific nucleosomes alongthe gene were modified. Time-course digestions of nuclei with micrococcal nuclease and DNaseI indicated that the enhancer/promoter region was more resistant to digestion in the inactive gene in pea roots than was the same region in the active gene in shoots, whereas the transcribed region of PetE was digested similarly among the tissues. This finding indicates that transcription is accompanied by changes in the nuclease accessibility of the enhancer/promoter region only. Moreover, these results indicate that the changes in nuclease accessibility are organ specific, whereas histone hyperacetylation is light dependent, and they suggest that changes in nuclease accessibility precede histone hyperacetylation during PetE activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 18:19:46