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Titolo:
Dynamic analyses of the expression of the HISTONE :: YFP fusion protein inarabidopsis show that syncytial endosperm is divided in mitotic domains
Autore:
Boisnard-Lorig, C; Colon-Carmona, A; Bauch, W; Hodge, S; Doerner, P; Bancharel, E; Dumas, C; Haseloff, J; Berger, F;
Indirizzi:
Univ Lyon 1, Ecole Natl Super Lyon, INRA, CNRS,Unite Mixte Rech 5667, F-69364 Lyon 07, France Univ Lyon 1 Lyon France 07 nite Mixte Rech 5667, F-69364 Lyon 07, France Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England Univ CambridgeCambridge England CB2 3EA Sci, Cambridge CB2 3EA, England Salk Inst Biol Studies, Plant Biol Lab, La Jolla, CA 92037 USA Salk Inst Biol Studies La Jolla CA USA 92037 Lab, La Jolla, CA 92037 USA
Titolo Testata:
PLANT CELL
fascicolo: 3, volume: 13, anno: 2001,
pagine: 495 - 509
SICI:
1040-4651(200103)13:3<495:DAOTEO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
GREEN FLUORESCENT PROTEIN; POLYCOMB GROUP GENE; CELL-DIVISION; TRANSGENIC PLANTS; SEED DEVELOPMENT; ROOT EPIDERMIS; WILD-TYPE; IN-VIVO; DROSOPHILA; THALIANA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Berger, F Univ Lyon 1, Ecole Natl Super Lyon, INRA, CNRS,Unite Mixte Rech 5667, F-69364 Lyon 07, France Univ Lyon 1 Lyon France 07 Rech 5667, F-69364 Lyon 07, France
Citazione:
C. Boisnard-Lorig et al., "Dynamic analyses of the expression of the HISTONE :: YFP fusion protein inarabidopsis show that syncytial endosperm is divided in mitotic domains", PL CELL, 13(3), 2001, pp. 495-509

Abstract

During early seed development, nuclear divisions in the endosperm are not followed by cell division, leading to the development of a syncytium. The simple organization of the Arabidopsis endosperm provides a model in which to study the regulation of the cell cycle in relation to development. To monitor nuclear divisions, we constructed a HISTONE 2B::YELLOW FLUORESCENT PROTEIN gene fusion (H2B::YFP). To validate its use as a vital marker for chromatin in plants, H2B::YFP was expressed constitutively in Arabidopsis. Thisenabled the observation of mitoses in living root meristems. H2B::YFP was expressed specifically in Arabidopsis syncytial endosperm by using GAL4 transactivation. Monitoring mitotic activity in living syncytial endosperm showed that the syncytium was organized into three domains in which nuclei divide simultaneously with a specific time course. Each mitotic domain has a distinct spatiotemporal pattern of mitotic CYCLIN B1;1 accumulation. The polar spatial organization of the three mitotic domains suggests interactions between developmental mechanisms and the regulation of the cell cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:44:05