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Titolo:
Control of the expression of human neuropeptide Y by leptin: in vitro studies
Autore:
Magni, P; Vettor, R; Pagano, C; Calcagno, A; Martini, L; Motta, M;
Indirizzi:
Univ Milan, Ist Endocrinol, Milan, Italy Univ Milan Milan ItalyUniv Milan, Ist Endocrinol, Milan, Italy Univ Padua, Dipartimento Sci Med & Chirurg, Padua, Italy Univ Padua Padua Italy ua, Dipartimento Sci Med & Chirurg, Padua, Italy
Titolo Testata:
PEPTIDES
fascicolo: 3, volume: 22, anno: 2001,
pagine: 415 - 420
SICI:
0196-9781(200103)22:3<415:COTEOH>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
FETAL BRAIN-CELLS; HUMAN NEUROBLASTOMA-CELLS; NERVE GROWTH-FACTOR; PHORBOL ESTER; GENE-EXPRESSION; MESSENGER-RNA; TRANSCRIPTIONAL REGULATION; OBESE GENE; RECEPTOR; NEURONS;
Keywords:
human neuroblastoma; gene expression; peptide secretion; leptin receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Magni, P Univ Milan, Ist Endocrinol, Milan, Italy Univ Milan Milan Italy niv Milan, Ist Endocrinol, Milan, Italy
Citazione:
P. Magni et al., "Control of the expression of human neuropeptide Y by leptin: in vitro studies", PEPTIDES, 22(3), 2001, pp. 415-420

Abstract

Neuropeptide Y (NPY) participates in the regulation of reproduction and food intake. The adipose-secreted hormone, leptin, has also been involved in these processes, and has been shown to exert its effects in part by controlling NPY synthesis and release at the hypothalamic level. In the present study, we utilized the SH-SY5Y human neuroblastoma cell line, to study the leptin-NPY interrelationships. SH-SY5Y cells were found to express leptin receptors (RT-PCR and Western blot analyses). A 24-h treatment with leptin at different concentrations did not affect NPY gene expression, but resulted in a stimulation of NPY release. This stimulated secretion was blocked by the combined treatment with leptin and the muscarinic agonist carbachol or the phorbol ester TPA. Leptin and carbachol also caused an increased intracellular content of NPY. In conclusion, the SH-SY5Y human neuroblastoma cell line appears to be a suitable in vitro model for studying the pharmacological effects of leptin on the biosynthesis and secretion of NPY. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 04:54:55