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Titolo:
Distribution and expression of trek-1, a two-pore-domain potassium channel, in the adult rat CNS
Autore:
Hervieu, GJ; Cluderay, JE; Gray, CW; Green, PJ; Ranson, JL; Randall, AD; Meadows, HJ;
Indirizzi:
SmithKline Beecham Pharmaceut, Neurosci Res, Harlow CM19 5AW, Essex, England SmithKline Beecham Pharmaceut Harlow Essex England CM19 5AW ssex, England
Titolo Testata:
NEUROSCIENCE
fascicolo: 4, volume: 103, anno: 2001,
pagine: 899 - 919
SICI:
0306-4522(2001)103:4<899:DAEOTA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYUNSATURATED FATTY-ACIDS; TWIK-1 K+ CHANNEL; BEHAVIORAL SENSITIZATION; HIPPOCAMPAL INTERNEURONS; FUNCTIONAL EXPRESSION; SUBUNIT; LOCALIZATION; APLYSIA; CLONING; MEMBER;
Keywords:
channel; brain; immunohistochemistry; interneurones; antiserum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Meadows, HJ SmithKline Beecham Pharmaceut, Neurosci Res, 3rd Ave, Harlow CM19 5AW, Essex, England SmithKline Beecham Pharmaceut 3rd Ave Harlow Essex England CM19 5AW
Citazione:
G.J. Hervieu et al., "Distribution and expression of trek-1, a two-pore-domain potassium channel, in the adult rat CNS", NEUROSCIENC, 103(4), 2001, pp. 899-919

Abstract

TREK-1 is a member of the two-pore-domain potassium channel family which is expressed predominantly in the CNS. Using an anti-peptide polyclonal antiserum, we have determined the distribution of TREK-1 in the brain and spinal cord of adult rats. Specificity of the antiserum was tested using a TREK-l-transfected cell line and confirmed with c-myctagged TREK-1. In thin tissue sections, immunoreactivity was widespread throughout the rat brain and spinal cord. TREK-1-like signals were observed in the cerebral cortex, basalganglia, hippocampus, and various other subcortical nuclei in the hypothalamus, thalamus, mesencephalon and rhombencephalon. TREK-1 labelling appeared to be over the entire cell membrane, including the cell body and processes. Cells that morphologically resembled projection neurones and interneurones but not glial cells were labelled. As interneurones and known GABAergic projection neurones were the predominant population labelled, we investigated the possibility that TREK-1 is expressed in GABA-containing neurones using; a specific anti-GABA antiserum. Expression of TREK-1 in GABA-containingneurones was observed in a number of areas: including the isocortex, hippocampus and thalamus. Thus, TREK-I expression defines a unique and specific subset of interneurones and principal cells. These studies indicate a widespread distribution of TREK-1 potassium channels throughout the rat brain and spinal cord, with expression in a number of areas being demonstrated to be present on GABA-containing neurones. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 04:53:13