Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Development and evaluation of an antibody capture ELISA for detection of IgG to Epstein-Barr virus in oral fluid samples
Autore:
Sheppard, C; Cohen, B; Andrews, N; Surridge, H;
Indirizzi:
Cent Publ Hlth Lab, Enter Resp & Neurol Virus Lab, London NW9 5HT, EnglandCent Publ Hlth Lab London England NW9 5HT s Lab, London NW9 5HT, England PHLS Stat Unit, London NW9 5EQ, England PHLS Stat Unit London England NW9 5EQ Stat Unit, London NW9 5EQ, England Univ E Anglia, Sch Hlth Policy & Practice, Norwich NR4 7TJ, Norfolk, England Univ E Anglia Norwich Norfolk England NR4 7TJ h NR4 7TJ, Norfolk, England
Titolo Testata:
JOURNAL OF VIROLOGICAL METHODS
fascicolo: 1-2, volume: 93, anno: 2001,
pagine: 157 - 166
SICI:
0166-0934(200104)93:1-2<157:DAEOAA>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
SALIVA;
Keywords:
Epstein-Barr virus; ELISA; oral fluid; mixture modelling; epidemiology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Sheppard, C Cent Publ Hlth Lab, Enter Resp & Neurol Virus Lab, 61 Colindale Ave, London NW9 5HT, England Cent Publ Hlth Lab 61 Colindale Ave London England NW9 5HT nd
Citazione:
C. Sheppard et al., "Development and evaluation of an antibody capture ELISA for detection of IgG to Epstein-Barr virus in oral fluid samples", J VIROL MET, 93(1-2), 2001, pp. 157-166

Abstract

The development of an EBV IgG antibody capture ELISA (GACELISA) for detection of EBV viral capsid antigen specific IgG in oral fluids is described. The assay was optimised and evaluated using paired serum and oral fluid samples from healthy laboratory staff (n = 82) and oral fluids collected eitherfor routine measles. mumps, and rubella testing (n = 629) or for an epidemiological study of atopic dermatitis (n = 252). Statistical analysis by mixture modelling was used to determine the GACELISA cut-off and to estimate the sensitivity and specificity of the assay. Sensitivity and specificity was also assessed by comparing the results of immunofluorescence assay for EBV specific IgG in serum with those of GACELISA in 82 matching oral fluids. Compared to serum immunofluorescence assay, oral fluid GACELISA was found to have a sensitivity of 82.2 and 88.9% specificity with these samples. Mixture modelling, predicted the GACELISA to be 88.4% sensitive and of 99.4% specific. The prevalence of antibody to EBV in oral fluids was found to be 73.8% in laboratory staff, 34.4-73.9% in measles, mumps, and rubella patientsand 22.2% in atopic dermatitis study participants. A robust. reliable and reproducible EBV GACELISA has been developed which will be a useful tool for epidemiological investigations. (C) 2001 Elsevier Science B.V. All rightsreserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:04:23