Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Optimisation and standardisation of a method for detecting and enumeratingbacteriophages infecting Bacteroides fragilis
Autore:
Araujo, R; Muniesa, M; Mendez, J; Puig, A; Queralt, N; Lucena, F; Jofre, J;
Indirizzi:
Univ Barcelona, Fac Biol, Dept Microbiol, E-08028 Barcelona, Spain Univ Barcelona Barcelona Spain E-08028 crobiol, E-08028 Barcelona, Spain
Titolo Testata:
JOURNAL OF VIROLOGICAL METHODS
fascicolo: 1-2, volume: 93, anno: 2001,
pagine: 127 - 136
SICI:
0166-0934(200104)93:1-2<127:OASOAM>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEWAGE;
Keywords:
anaerobiosis; Bacteroides fragilis; method; phages;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Jofre, J Univ Barcelona, Fac Biol, Dept Microbiol, Diagonal 645, E-08028 Barcelona,Spain Univ Barcelona Diagonal 645 Barcelona Spain E-08028 celona,Spain
Citazione:
R. Araujo et al., "Optimisation and standardisation of a method for detecting and enumeratingbacteriophages infecting Bacteroides fragilis", J VIROL MET, 93(1-2), 2001, pp. 127-136

Abstract

A method for the detection and enumeration of bacteriophages infecting Bacteroides fragilis has been standardised. The recommended host strain is RYC2056 (ATCC 700786) because of the relatively high counts (10(4)-10(5) PFU/100ml) that it recovers in sewage from very different geographical areas. The addition of 0.25% bile to the culture and assay media and the manipulation of the host strain under strict anaerobic conditions resulted in a significant increase (more than 100%) in the number of phages detected, No other changes in the media and culture conditions resulted in changes in the phage counts detected. However, these increases do not justify changing the culture conditions and media described, taking into consideration that bile renders the media cloudy making it difficult to follow the host growth and that most laboratories do not have the: facilities to work under strict anaerobic conditions. Nalidixic acid (100 mug/ml) and kanamycin (100 mug/ml) in the assay medium significantly reduce the background flora from polluted samples without affecting the phage counts. Freezing cultures just before theend of the log-phage growth at ( -70 +/- 10)degreesC with BSA-sucrose as cryoprotector, storing of 1-2 mi in glass vials at (-70 +/- 10)degreesC and using them directly to inoculate fresh broth allows the obtention of cultures ready for phage enumeration in about 2.5 h. All these developments have been incorporated into a procedure that makes the method for detecting phages infecting B. fragilis as workable as the standardised methods available for the detection of coliphages. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 18:03:47