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Titolo:
Understanding chemistry of spermatozoa using NMR
Autore:
Govil, G; Srivastava, S;
Indirizzi:
Tata Inst Fundamental Res, Bombay 400005, Maharashtra, India Tata Inst Fundamental Res Bombay Maharashtra India 400005 arashtra, India
Titolo Testata:
JOURNAL OF THE INDIAN CHEMICAL SOCIETY
fascicolo: 3, volume: 78, anno: 2001,
pagine: 103 - 111
SICI:
0019-4522(200103)78:3<103:UCOSUN>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; EJACULATED RABBIT SPERMATOZOA; SEMINAL PLASMA; INTRACELLULAR PH; INVITRO CAPACITATION; P-31-NMR ANALYSIS; GOAT SPERMATOZOA; SPERM MOTILITY; SPECTROSCOPY; BOVINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Govil, G Tata Inst Fundamental Res, Bombay 400005, Maharashtra, India TataInst Fundamental Res Bombay Maharashtra India 400005 India
Citazione:
G. Govil e S. Srivastava, "Understanding chemistry of spermatozoa using NMR", J INDIAN CH, 78(3), 2001, pp. 103-111

Abstract

In recent years, NMR has found wide applications, on one end to study the structure of molecules and on the other, to study intact body organs such as brain or heart. In between these two extremes is another fascinating branch of science, namely, the study of chemistry of intact cellular systems. White dedicated instruments for this type of work are not available, it is possible to use high field high resolution NMR spectrometers meant for molecular studies (e.g. a 300-500 MHz instrument) to obtain chemical and biological information on living cells. We have studied the chemistry of one of the important cellular systems, spermatozoa, using NMR. Spermatozoa play a key role in reproductive biology. Using techniques such as HSQC, it has been possible to obtain information on the H-1 end C-13 NMR features of such cells. We have been able to detect a number of molecules in these sperm cells,which had evaded detection by conventional chemical tools. It has been possible to follow chemical pathways and kinetics of metabolism using conventional C-13 NMR. P-31 NMR provides valuable information on the energetics andpH profiles. Some of these studies have helped us to know how such cells can be activated or deactivated. For example, arginine deficiency is known to cause diseases such as oligospermia and asthenospermia. NMR experiments have established that the glycolysis and fructolysis of spermatozoa is activated by arginine. Arginine also increases synthesis of ATP, which is essential for sperm motility. The chemical information thus obtained has major biological implication for storage, activation and control of spermatozoa.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 15:08:22