Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Comparison of cloned Kir2 channels with native inward rectifier K+ channels from guinea-pig cardiomyocytes
Autore:
Liu, GX; Derst, C; Schlichthorl, G; Heinen, SN; Seebohm, G; Bruggemann, A; Kummer, W; Veh, RW; Daut, J; Preisig-Muller, R;
Indirizzi:
Univ Marburg, Inst Normale & Pathol Physiol, D-35037 Marburg, Germany UnivMarburg Marburg Germany D-35037 l Physiol, D-35037 Marburg, Germany Aventis, Cardiovasc Res, D-65926 Frankfurt, Germany Aventis Frankfurt Germany D-65926 iovasc Res, D-65926 Frankfurt, Germany Univ Giessen, Inst Anat & Zellbiol, D-35385 Giessen, Germany Univ GiessenGiessen Germany D-35385 Zellbiol, D-35385 Giessen, Germany Charite, Inst Anat, D-10098 Berlin, Germany Charite Berlin Germany D-10098 arite, Inst Anat, D-10098 Berlin, Germany
Titolo Testata:
JOURNAL OF PHYSIOLOGY-LONDON
fascicolo: 1, volume: 532, anno: 2001,
pagine: 115 - 126
SICI:
0022-3751(20010401)532:1<115:COCKCW>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECTIFYING POTASSIUM CHANNELS; FUNCTIONAL EXPRESSION; MOLECULAR-CLONING; CARDIAC MYOCYTES; SODIUM-CHANNELS; XENOPUS-LAEVIS; SINGLE-CHANNEL; HUMAN ATRIUM; HEART; BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Daut, J Univ Marburg, Inst Normale & Pathol Physiol, Deutschhausstr 2, D-35037 Marburg, Germany Univ Marburg Deutschhausstr 2 Marburg Germany D-35037 rg, Germany
Citazione:
G.X. Liu et al., "Comparison of cloned Kir2 channels with native inward rectifier K+ channels from guinea-pig cardiomyocytes", J PHYSL LON, 532(1), 2001, pp. 115-126

Abstract

1. The aim of the study was to compare the properties of cloned Kir2 channels with the properties of native rectifier channels in guinea-pig (gp) cardiac muscle. The cDNAs of gpKir2.1, gpKir2.2, gpKir2.3 and gpKir2.4 were obtained by screening a cDNA library from guinea-pig cardiac ventricle.2. A partial genomic structure of all gpKir2 genes was deduced by comparison of the cDNAs with the nucleotide sequences derived from a guinea-pig genomic library.3. The cell-specific expression of Kir2 channel subunits was studied in isolated cardiomyocytes using a multi-cell RT-PCR approach. It was found thatgpKir2.1, gpKir2.2 and gpKir2.3, but not gpKir2.3, are expressed in cardiomyocytes.4. Immunocytochemical analysis with polyclonal antibodies showed that expression of Kir2.4 is restricted to neuronal cells in the heart.5. After transfection in human embryonic kidney cells (HEK293) the mean single-channel conductance with symmetrical K+ was found to be 30.6 pS for gpKir2.1, 40.0 pS for gpKir2.2 and 14.2 pS for Kir2.3.6. Cell-attached measurements in isolated guinea-pig cardiomyocytes (n = 351) revealed three Populations of inwardly rectifying K+ channels with meanconductances of 34.0, 23.8 and 10.7 pS.7. Expression of the gpKir2 subunits in Xenopus oocytes showed inwardly rectifying currents. The Ba2+ concentrations required for half-maximum block at -100 mV were 3.24 muM for gpKir2.1, 0.51 muM for gpKir2.2, 10.26 muM forgpKir2.3 and 235 muM for gpKir2.4.8. Ba2+ block of inward rectifier channels of cardiomyocytes n as studied in cell-attached recordings. The concentration and voltage dependence of Ba2+ block of tile large-conductance inward rectifier channels was virtually identical to that of gpKir2.2 expressed in Xenopus oocytes.9. Our results suggest that the large-conductance inward rectifier channels found in guinea-pig cardiomycocytes (34.0 pS) correspond to gpKir2.2. Theintermediate-conductance (23.8 pS) and low-conductance (10.7 pS) channels described here may correspond to gpKir2.1 and gpKir2.3, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 17:42:09